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Etude de Pan A et de Pan B : deux protéines régulatrices du protéasome chez les archaea halophiles.

Abstract : Proteasomes are large ATP-dependent proteases implicated in degradation of key regulatory cellular proteins and abnormal cellular proteins. The current work is the first characterization of two AAA-ATPase proteasomal regulatory proteins PAN A and PAN B in halophilic archaea. The first part of the manuscript characterizes the mode of regulation of the two PANs in the extremely halophilic archaoen Halobacterium salinarium. Our experiments show the existence of two isoforms of each PAN proteins in the cells. The two isoforms differ in their N-terminal regions and result probably from the use of two alternative transcription start sites. Primer extension methods demonstrate the existence of heterogeneity in PAN's mRNA as revealed by mapping of the 5' non coding regions. The second part of the manuscript shed lights on oligomerisation and association of the PAN proteins in cellulo. Fractionation of cell free extracts on sucrose gradients indicates a weak degree of oligomerisation of the PANs and the absence of stable complexes with the proteasome 20 S. The last part of this work focuses on the regulation of PAN A and PAN B gene expression in response to stress including heat shock and salt shock. Our results clearly show that the two proteasome activating proteins are not regulated in a similar way in response to environmental stress
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Contributor : Hala Chamieh <>
Submitted on : Friday, January 27, 2006 - 9:20:40 AM
Last modification on : Tuesday, October 6, 2020 - 4:12:03 PM
Long-term archiving on: : Tuesday, April 6, 2010 - 4:41:28 PM


  • HAL Id : tel-00011475, version 1



Hala Chamieh. Etude de Pan A et de Pan B : deux protéines régulatrices du protéasome chez les archaea halophiles.. Biochimie [q-bio.BM]. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00011475⟩



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