Skip to Main content Skip to Navigation
Theses

Canaux calciques des spermatozoïde de Mammifères
Caractérisation des interactions fonctionnelles et moléculaires au cours de la réaction acrosomique

Abstract : The acrosome reaction in mouse is triggered by a long lasting calcium signaling produced by a chain of openings of several calcium channels, a low voltage-activated (LVA) calcium channel, an inositol trisphosphate receptor (IP3R) and the store-operated calcium channel TRPC2. Our data evidence an interesting functional relationship, in this cell type, between LVA channels and proteins whose activity is related to calcium filling state of the endoplasmic reticulum (presumably TRP channels and Inositol triphosphate Receptor). All the calcium signalling is under the control of the activation of the first-one, a T-type calcium channel. Using mice deficient for CaV3.1 and subunit CaV3.2, we show that CaV3.2 represent the main functional channels in wild-type spermatogenic cells.Our results provide a new hypothesis concerning sperm TRPC2 gating: TRPC2 activation may be due to modifications of its interaction with both junctate and IP3R, induced by acrosome depletion.
Document type :
Theses
Complete list of metadatas

Cited literature [199 references]  Display  Hide  Download

https://tel.archives-ouvertes.fr/tel-00011465
Contributor : Severine Stamboulian <>
Submitted on : Wednesday, January 25, 2006 - 6:07:02 PM
Last modification on : Friday, November 6, 2020 - 4:07:15 AM
Long-term archiving on: : Monday, September 17, 2012 - 11:11:03 AM

Identifiers

  • HAL Id : tel-00011465, version 1

Collections

INSERM | UJF | CEA | UGA

Citation

Severine Stamboulian. Canaux calciques des spermatozoïde de Mammifères
Caractérisation des interactions fonctionnelles et moléculaires au cours de la réaction acrosomique. Biologie de la reproduction. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00011465⟩

Share

Metrics

Record views

445

Files downloads

2667