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Génération et mécanismes d'action anti-tumorale d'effecteurs lymphocytaires T CD4+ dans les lymphomes B malins     

Abstract : The importance of CD4+ T lymphocytes in anti-tumor immune responses has been increasingly highlighted in the course of those last ten years. We have studied their function in B-cell non-Hodgkin lymphomas.
In the first part of this thesis, we have studied a model derived from fresh splenic cells of a patient with a splenic marginal-zone B cell lymphoma. We have been able to demonstrate a functional effect of reactive CD4+ T lymphocytes onto autologous malignant B cells, that is, the CD4+ T cell-induced differentiation of the autologous malignant B cells into plasmocytes.
We have then studied another aspect of the functionality of reactive CD4+ T cells towards a follicular B cell lymphoma line obtained in our laboratory. Total autologous CD4+ T cells from the peripheral blood lymphocytes were cytotoxic to autologous malignant B cells, as well as normal lymphoblastic cells. However, cloning of the reactive CD4+ T cells further identified three clones with specific cytotoxicity against malignant cells. All three clones possess an identical TCR Vb17-Db1-Jb1.2, are HLA-DP restricted, and they perform cytolysis through a classical perforin/granzyme pathway.
These results allow us concluding that CD4+ T cells can induce a direct anti-tumor effect with multifarious mechanisms. This work provides novel arguments in favor of the pivotal role of CD4+ T lymphocytes in the anti-tumor immunity, as well as a methodology for further identification of tumor antigens in B cell lymphomas.     
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https://tel.archives-ouvertes.fr/tel-00011308
Contributor : Jian-Qing Mi <>
Submitted on : Friday, January 6, 2006 - 2:35:32 AM
Last modification on : Friday, November 6, 2020 - 3:46:01 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 4:35:19 PM

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  • HAL Id : tel-00011308, version 1

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Jian-Qing Mi. Génération et mécanismes d'action anti-tumorale d'effecteurs lymphocytaires T CD4+ dans les lymphomes B malins     . Immunologie. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00011308⟩

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