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Identification de gènes impliqués dans le développement des lymphones cutanés primitifs CD30+

Abstract : The CD30+ Primary Cutaneous Lymphoma (PCL) is a malignant T-cells lymphoproliferation. Its oncogenesis remains currently unknown. The aim of my thesis is to identify genes expressed specifically in CD30+ PCL in order to understand the mechanisms of oncogenesis and also to identify diagnostic or therapeutics markers. The subtracted libraries of cDNA are obtained by SSH “suppressive subtractive hybridisation” between CD30+ PCL and blood lymphocytes. These libraries are then screened by dot blot techniques with specific radiolabelled probes of the studied samples. The selected clones are finally validated by real time PCR. We have thus found five genes over expressed in CD30+ PCL: THW, p120catenin, SPARC, PTTG1, and CD30 and five genes under expressed: humanin, CIN85, Bcl11B, CD30L and CD30s. It remains to be checked if these genes are responsible for oncogenesis or if they are uniquely phenotypes of CD30+ PCL.
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https://tel.archives-ouvertes.fr/tel-00011307
Contributor : Emilie Loreau <>
Submitted on : Friday, February 10, 2006 - 11:24:59 AM
Last modification on : Wednesday, November 29, 2017 - 10:02:36 AM
Long-term archiving on: : Saturday, April 3, 2010 - 8:55:23 PM

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  • HAL Id : tel-00011307, version 1

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Emilie Loreau. Identification de gènes impliqués dans le développement des lymphones cutanés primitifs CD30+. Immunologie. Université Victor Segalen - Bordeaux II, 2003. Français. ⟨tel-00011307⟩

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