Skip to Main content Skip to Navigation
Theses

Etude de l'action bioprotectrice des sucres : une investigation par dynamique moléculaire et spectroscopie Raman

Abstract : Understanding the enhanced efficiency of trehalose to stabilise biological
molecules compared to other excipients is a matter of intense research. We
have studied the influence of trehalose and two other stereoisomers, maltose
and sucrose, on the structure and on the dynamics of water and of a model
globular protein, lysozyme. We have shown that trehalose induces a greater
destructuration of the water hydrogen bond (HB) network than maltose and
sucrose, beyond a concentration of 40-50 wt %, at which the HB network of
sugars percolates. Moreover, the hydration number of sugars and the number of
sugar-sugar HBs suggest that trehalose aqueous solutions are more "homogeneous", for concentrations between 33 and 66 wt %. We have also
localised interaction zones between sugars and lysozyme and shown with
Raman spectroscopy that trehalose stabilises it more.
Complete list of metadatas

https://tel.archives-ouvertes.fr/tel-00011225
Contributor : Adrien Lerbret <>
Submitted on : Friday, December 16, 2005 - 6:16:20 PM
Last modification on : Monday, October 19, 2020 - 2:26:01 PM
Long-term archiving on: : Saturday, April 3, 2010 - 7:30:46 PM

Identifiers

  • HAL Id : tel-00011225, version 1

Collections

Citation

Adrien Lerbret. Etude de l'action bioprotectrice des sucres : une investigation par dynamique moléculaire et spectroscopie Raman. Biophysique [physics.bio-ph]. Université des Sciences et Technologie de Lille - Lille I, 2005. Français. ⟨tel-00011225⟩

Share

Metrics

Record views

261

Files downloads

939