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Modélisation d'expériences permettant la manipulation de molécules biologiques uniques

Abstract : Modelization of mechanisms regulating gene expression is the main topic of this manuscript which is divided in two parts.
Looping of the DNA molecule is studied from the static point of view. Size of proteins clamping the loop is taken into account as well as mechanisms inducing stiffness loss - additional proteins binding for instance - or stretching of the molecule thanks to an external force. End-to-end distance distributions including the cyclization factor of the "Worm-Like Chain" (WLC) have been computed using numerical methods. This semiflexible polymer model includes only bending stiffness all along this work. The analytical expression Shimada and Yamakawa derived in 1984 is furthermore extended.
Follows a brief report on numerical stochastic algorithms which allow the dynamical simulation of the plasmid ColE1 Copy Number Control (CNC) mechanism. These should shortly complement futher theoretical and experimental studies on robustness with respect to noise.
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https://tel.archives-ouvertes.fr/tel-00011190
Contributor : Nicolas Douarche <>
Submitted on : Monday, December 12, 2005 - 6:59:51 PM
Last modification on : Thursday, December 10, 2020 - 12:36:53 PM
Long-term archiving on: : Saturday, April 3, 2010 - 6:53:35 PM

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  • HAL Id : tel-00011190, version 1

Citation

Nicolas Douarche. Modélisation d'expériences permettant la manipulation de molécules biologiques uniques. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2005. Français. ⟨tel-00011190⟩

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