Électrochromatographie capillaire. Evaluation d'une nouvelle phase stationnaire mixte et application à l'analyse de peptides et à l'établissement de cartes peptidiques

Abstract : A new embedded stationary phase, with quaternary ammonium and octadecyl chain, has been investigated in capillary electrochromatography (CEC). I have developed a process to pack the capillaries with such a phase. The electroosmotic flow (EOF), peak efficiency and a new parameter: the real electrokinetic porosity have demonstrated the presence of perfusive EOF. The latter parameter appears to be also a good evaluator of stationary phase wettability. I have estimated chromatographic retention in CEC for peptides in acidic conditions and demonstrated the good ability of mixed mode stationary phase for peptide analysis and peptide mapping. A comparison HPLC/CEC has indicated a strong difference of chromatographic retention mode between these two analytical modes and the original chromatographic behaviour in CEC. In our conditions, electrophoretic mechanisms played a major role in CEC. Although no electrostatic exclusion could be evidenced, retention by reversed phase retention was quite low.
Document type :
Theses
Other. Université Paris Sud - Paris XI, 2005. French


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Submitted on : Sunday, November 6, 2005 - 7:02:14 PM
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  • HAL Id : tel-00010881, version 1

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Frédéric Progent. Électrochromatographie capillaire. Evaluation d'une nouvelle phase stationnaire mixte et application à l'analyse de peptides et à l'établissement de cartes peptidiques. Other. Université Paris Sud - Paris XI, 2005. French. <tel-00010881>

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