GALIG : UN NOUVEAU GÈNE HUMAIN INDUCTEUR DE LA MORT CELLULAIRE

Abstract : Galig, an internal gene emedded in the galectin-3 gene, encodes two proteins: mitogaligin and cytogaligin. This work shows that galig expression leads to cell death with specific characteristics of apoptosis. Indeed, Bcl-XL co-expression, an anti-apoptotic protein, significantly reduced the release of cytochromec and cell death. Nevertheless, various apoptotic markers are not point out suggesting a novel form of programmed cell death. Structure-activity relationship studies showed that the mitochondrial addressing of mitogaligin relies on an internal sequence. Polyclonal anti-cytogaligin antibodies have been produced and used in immunofluorescence and western-blot. Quantitative PCR test has also been set up. This tools will allowed to quantify galig gene expression and in vivo proteins production in several human tissues.
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https://tel.archives-ouvertes.fr/tel-00010769
Contributor : Patricia Leloup <>
Submitted on : Wednesday, October 26, 2005 - 11:51:24 AM
Last modification on : Wednesday, June 27, 2018 - 8:24:02 AM
Long-term archiving on : Friday, April 2, 2010 - 11:03:23 PM

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  • HAL Id : tel-00010769, version 1

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Mélanie Duneau. GALIG : UN NOUVEAU GÈNE HUMAIN INDUCTEUR DE LA MORT CELLULAIRE. domain_other. Université d'Orléans, 2005. Français. ⟨tel-00010769⟩

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