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Theses

LES GÈNES DE TYPE FIBRILLINE D'ARABIDOPSIS THALIANA, FONCTION ET RÉGULATION

Abstract : Fibrillin is one of the major proteins in fibrillar carotenoid storage structures of bell pepper chromoplasts. In order to understand better the function of this protein, we studied the fibrillin homologues in the Arabidopsis thaliana plant model. We have identified 13 genes encoding members of this family in Arabidopsis thaliana which form 10 groups. As proteins of the fibrillin type are also present in other plants and in cyanobacteria, we propose that during evolution, plants multiplied their fibrillin genes from an ancestral gene that still has a relative in some cyanobacteria. In Arabidopsis thaliana, the 13 FIB genes show differential expression when stress- or organ-related expression is examined. We focused on the FIB1-2 subfamily which is the closest to the bell pepper fibrillin that was first characterised. FIB1-2 subfamily genes show a developmental regulation with a good induction in leaves and in flowers. The FIB1b protein accumulates in these organs, suggesting a transcriptional regulation. FIB1a and FIB1b are also induced under stress conditions like the bell pepper FIB gene, whereas FIB2 is more constitutively expressed. The involvement of the fibrillin gene in stress response has been studied in fibrillin RNAi plants which repress several members of this gene family. These plants showed a growth delay, but no drastic modification in response to stress conditions. Finally, we have developed a mutagenesis approach in order to dissect the regulation pathway leading to the bell pepper FIB gene expression. We have produced and screened an EMS mutagenised Arabidopsis thaliana population carrying a double reporter gene construct (LUC, GUS) driven by the bell pepper fibrillin promoter. We isolated mutants altered in the regulation of the fibrillin promoter in flowers.
Mots-clés : Caroténoides stress
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https://tel.archives-ouvertes.fr/tel-00010436
Contributor : Yec'Han Laizet <>
Submitted on : Thursday, October 6, 2005 - 9:48:45 AM
Last modification on : Friday, November 6, 2020 - 3:57:20 AM
Long-term archiving on: : Friday, April 2, 2010 - 10:29:38 PM

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  • HAL Id : tel-00010436, version 1

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Yec'Han Laizet. LES GÈNES DE TYPE FIBRILLINE D'ARABIDOPSIS THALIANA, FONCTION ET RÉGULATION. Biologie végétale. Université Joseph-Fourier - Grenoble I, 2005. Français. ⟨tel-00010436⟩

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