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Hypermutabilité et adaptation chez les souches de Staphylococcus aureus isolées de mucoviscidose : rôle des gènes mutS et mutL et impact sur la résistance aux macrolides

Abstract : Our observation that S. aureus strains isolated from cystic fibrosis were more frequently hypermutable than those isolated in other pathologies led us to explore the mechanisms underlying hypermutability in this species. Alterations of the mismatch repair system genes, mutS and mutL, accounted for the hypermutable phenotype of four clinical strains. We showed that both genes were cotranscribed in S. aureus and that inactivation of either gene resulted in an hypermutable phenotype. We designed a model to study the role of these genes in recombination in S. aureus, which seemed to show that both only have a limited role in the phenomenon in this species. Hypermutability has also an impact on antibiotic resistance of the strains: macrolide resistance is more and more frequent in S. aureus strains isolated from cystic fibrosis, and we showed that, unexpectedly, it could not be attributed to transposon or plasmid-borne resistance genes, but that it was mostly due to mutations in the ribosomal target of these antibiotics. We thus described mutations in the following genes: rrl (encoding ribosomal RNA), rplD and rplV (encoding ribosomal proteins L4 and L22, respectively).
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https://tel.archives-ouvertes.fr/tel-00009534
Contributor : Anne-Laure Prunier <>
Submitted on : Sunday, June 19, 2005 - 10:01:47 PM
Last modification on : Tuesday, February 5, 2019 - 12:12:27 PM
Long-term archiving on: : Friday, April 2, 2010 - 10:36:50 PM

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  • HAL Id : tel-00009534, version 1

Citation

Anne-Laure Prunier. Hypermutabilité et adaptation chez les souches de Staphylococcus aureus isolées de mucoviscidose : rôle des gènes mutS et mutL et impact sur la résistance aux macrolides. Biochimie [q-bio.BM]. Université de Caen, 2004. Français. ⟨tel-00009534⟩

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