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Uniaxial Mechanical Assays on Adherent Living Single Cells : Animal Embryonic Fibroblasts and Human Pancreas Cancer Cells

Abstract : Despite their biological complexity, animals, living tissues and cells, the smallest unit of live, are subjected to physical principles. In living tissues, permanent or transient regulation processes, such as biochemical and mechanical interactions between a single cell and its environment are essential to develop tissue structure and function. These interactions play a major role in biological processes such as differentiation and gene expression of cells. In contrast, metastatic cancer cells escape such regulations. These cells proliferate and migrate through tissues unregulated, ignoring input from environment. Their mechanical and adhesion properties are drastically different than those of non-tumour cells. The presented studies describe experiments which mimic conditions in vivo by applying uniaxial stress to a single living cell under physiological conditions. Simultaneously, applied force, cell deformation and cellular shape are determined with highest accuracy. Uniaxial deformation is applied to a single cell which adheres between two parallel glass plates. Such mechanical assays accomplished at constant displacement or constant force allow for quantifying cellular viscoelastic properties and for describing the data by using linear force relations or the Kelvin model. Biolipids such as sphingosylphosphorylcholine and lysophosphatidic acid modulated the cytoskeleton architecture which showed to cause fatal consequences for mechanical properties of rat embryonic fibroblasts and human pancreatic cancer cells.
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Contributor : Alexandre Micoulet <>
Submitted on : Friday, June 17, 2005 - 4:46:29 PM
Last modification on : Friday, November 6, 2020 - 3:53:12 AM
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  • HAL Id : tel-00009530, version 1




Alexandre Micoulet. Uniaxial Mechanical Assays on Adherent Living Single Cells : Animal Embryonic Fibroblasts and Human Pancreas Cancer Cells. Biological Physics []. Université Joseph-Fourier - Grenoble I, 2004. English. ⟨tel-00009530⟩



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