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controle de la transition meiose I/meiose II et role de DOC1R au cours de l'arret CSF lors de la maturation meiotique chez la souris

Abstract : Meiotic maturation of vertebrate oocytes differs from mitosis on many aspects. I was interested in two characteristics. 1) In meiosis I, homologous chromosomes are segregated, in mitosis sister chromatids are separated. In mitosis, a checkpoint blocks the cell in metaphase via APC/C inhibition until all chromosomes are properly aligned on the spindle. In meiosis, contradictory results exist, depending on the species, about the requirement of such a checkpoint in meiosis I. I have shown that separase activity (an activity indirectly regulated by APC/C) is required for metaphase to anaphase transition in meiosis I, suggesting that such a checkpoint is required in the mouse, an organism close of human. 2) At the end of meiotic maturation, oocytes are blocked in metaphase of meiosis II waiting for fertilization, whereas mitosis always ends. This block is due to a CSF activity and requires the Mos/.../MAPK pathway. I have shown that DOC1R, a new MAPK substrate, controls microtubule organization during the CSF arrest. These results establish a new view of the CSF arrest which was considered as a linear pathway responsible for MPF stabilization. The CSF arrest is a non linear pathway which also controls oocyte morphology.
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Contributor : Marie-Emilie Terret <>
Submitted on : Thursday, June 9, 2005 - 6:26:11 PM
Last modification on : Saturday, December 12, 2020 - 3:28:31 AM
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Marie-Emilie Terret. controle de la transition meiose I/meiose II et role de DOC1R au cours de l'arret CSF lors de la maturation meiotique chez la souris. Biologie cellulaire. Université Pierre et Marie Curie - Paris VI, 2004. Français. ⟨tel-00009435⟩

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