H. Hz, J. Ar-;-t, H. Hz, and . Ar, 3 Hz ; H Ar ) ppm (2H ; t, Per-CH 2, Hz, -CH 2 CH 2 CH 2 -), pp.99-100

. Le-iodure-de, 452 mg ; 1,4 mmol 1eq.) et le bromure de N-(5- carboxypentyl)lépidinium (474 mg; 1,4 mmol; 1eq.) sont dissous dans 10 mL d'un mélange MeOH:1, v/v) dans un ballon. On ajoute 150 µL de TEA, la solution prend immédiatement une couleur rouge sombre, Après une nuit, le mélange r nnel est concentré à sec et purifié sur colonne de silice (éluant: MeOH/CH 2 Cl, pp.3-5

J. D. Watson and . H. Crickf, Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid, Nature, vol.9, issue.4356, pp.737-738, 1953.
DOI : 10.1016/0006-3002(53)90232-7

G. Felsenfeld, D. R. Davies, and A. Rich, FORMATION OF A THREE-STRANDED POLYNUCLEOTIDE MOLECULE, Journal of the American Chemical Society, vol.79, issue.8, pp.2023-2024, 1957.
DOI : 10.1021/ja01565a074

J. Marmur and P. Doty, Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature, Journal of Molecular Biology, vol.5, issue.1, pp.109-118, 1962.
DOI : 10.1016/S0022-2836(62)80066-7

J. Mergny and L. Lacroix, Analysis of Thermal Melting Curves, Oligonucleotides, vol.13, issue.6, pp.515-537, 2003.
DOI : 10.1089/154545703322860825

R. L. Letsinger, J. L. Finnan, and W. B. Lundsford, Nucleotide chemistry. XX. Phosphite coupling procedure for generating internucleotide links, Journal of the American Chemical Society, vol.97, issue.11, pp.3278-3279, 1975.
DOI : 10.1021/ja00844a090

P. Kumar, S. Mahajan, and K. C. Gupta, Universal Reusable Polymer Support for Oligonucleotide Synthesis, The Journal of Organic Chemistry, vol.69, issue.19, pp.6482-6485, 2004.
DOI : 10.1021/jo049575v

F. Seela and U. Kretschmer, Diastereomerically pure Rp and Sp dinucleoside H-phosphonates: the stereochemical course of their conversion into P-methylphosphonates, phosphorothioates, and [oxygen-18] chiral phosphates, The Journal of Organic Chemistry, vol.56, issue.12, pp.3861-3869, 1991.
DOI : 10.1021/jo00012a017

X. Part, R. H. Hall, A. R. Todd, and R. F. Webb, Mixed anhydrides as intermediates in the synthesis of dinucleosides phosphates, J. Chem. Soc, pp.3291-3296, 1957.

T. J. Metray and E. T. Kool, Selective and Stable DNA Base Pairing without Hydrogen Bonds, Journal of the American Chemical Society, vol.120, issue.24, pp.6191-6192, 1998.
DOI : 10.1021/ja9803310

V. A. Korshun, N. B. Pestov, K. R. Birikh, and Y. A. Berlin, Reagent for introducing pyrene residues in oligonucleotides, Bioconjugate Chemistry, vol.3, issue.6, pp.559-562, 1992.
DOI : 10.1021/bc00018a016

S. I. Khan and M. W. Grinstaff, Palladium (0)-catalysed modification of oligonucleotides during automated solid-phase synthesis, J. Am. Chem. Soc, vol.121, pp.47704-4705, 1999.

J. D. Kahl and M. M. Greenberg, Introducing Structural Diversity in Oligonucleotides via Photolabile, Convertible C5-Substituted Nucleotides, Journal of the American Chemical Society, vol.121, issue.4, pp.597-604, 1999.
DOI : 10.1021/ja983273r

D. Miller, G. P. Kool, and E. T. , A Simple Method for Electrophilic Functionalization of DNA, Organic Letters, vol.4, issue.21, pp.3599-3601, 2002.
DOI : 10.1021/ol0264915

G. P. Miller and E. T. Kool, Versatile 5???-Functionalization of Oligonucleotides on Solid Support:?? Amines, Azides, Thiols, and Thioethers via Phosphorus Chemistry, The Journal of Organic Chemistry, vol.69, issue.7, pp.2404-2410, 2004.
DOI : 10.1021/jo035765e

K. J. Gibson and S. J. Benkovic, Synthesis and application of derivatizable oligonucleotides, Nucleic Acids Research, vol.15, issue.16, pp.6455-6467, 1987.
DOI : 10.1093/nar/15.16.6455

S. Agrawal and J. Tang, Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling, Tetrahedron Letters, vol.31, issue.11, pp.1543-1546, 1990.
DOI : 10.1016/0040-4039(90)80011-A

S. Agrawal, Methos in molecular biology, 1994.

H. Salo, P. Virta, H. Hakala, T. P. Prakash, A. M. Kawazaki et al., Aminooxy Functionalized Oligonucleotides:?? Preparation, On-Support Derivatization, and Postsynthetic Attachment to Polymer Support, Bioconjugate Chemistry, vol.10, issue.5, pp.815-823, 1999.
DOI : 10.1021/bc990021m

E. Defrancq and J. Lhomme, Use of an aminooxy linker for the functionalization of oligodeoxyribonucleotides, Bioorganic & Medicinal Chemistry Letters, vol.11, issue.7, pp.931-933, 2001.
DOI : 10.1016/S0960-894X(01)00108-1

B. A. Connolly and P. Rider, Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes, Nucleic Acids Research, vol.13, issue.12, pp.4485-4502, 1985.
DOI : 10.1093/nar/13.12.4485

J. A. Burns, J. C. Butler, J. Moran, and G. M. Whitesides, Selective reduction of disulfides by tris(2-carboxyethyl)phosphine, The Journal of Organic Chemistry, vol.56, issue.8, pp.2648-2650, 1991.
DOI : 10.1021/jo00008a014

G. N. Grimm, A. S. Boutorine, and C. Hélène, Rapid Routes of Synthesis of Oligonucleotide Conjugates from Non-Protected Oligonucleotides and Ligands Possessing Different Nucleophilic or Electrophilic Functional Groups, Nucleosides, Nucleotides and Nucleic Acids, vol.27, issue.10-12, pp.1943-1965, 2000.
DOI : 10.1093/nar/14.20.7985

E. Kostenko, M. Dobrikov, D. Pyshnyi, V. Petyuk, N. Komarova et al., 5'-bis-pyrenylated oligonucleotides displaying excimer fluorescence provide sensitive probes of RNA sequence and structure, Nucleic Acids Research, vol.29, issue.17, pp.3611-3620, 2001.
DOI : 10.1093/nar/29.17.3611

E. Kostenko, M. Donrikov, N. Komarova, D. Pyshniy, V. Vlassov et al., 5???-BIS-PYRENYLATED OLIGONUCLEOTIDES DISPLAY ENHANCED EXCIMER FLUORESCENCE UPON HYBRIDIZATION WITH DNA AND RNA, Nucleosides, Nucleotides and Nucleic Acids, vol.111, issue.10-11, pp.1859-1870, 2001.
DOI : 10.1081/NCN-100107197

L. Doan, T. Perrouault, L. Asseline, U. Thuong, N. T. Rivalle et al., Recognition and Photo-Induced Cleavage and Cross-Linking of Nucleic Acids by Oligonucleotides Covalently Linked to Ellipticine, Antisense Research and Development, vol.1, issue.1, pp.43-54, 1991.
DOI : 10.1089/ard.1991.1.43

D. Lyttle, M. H. Adams, H. Hudson, D. Cook, and R. M. , Versatile Linker Chemistry for Synthesis of 3???-Modified DNA, Bioconjugate Chemistry, vol.8, issue.2, pp.193-198, 1997.
DOI : 10.1021/bc970010y

L. E. Velea, Intercalative binding of small molecules to nucleic acids Graves D, Curr. Org. Chem, vol.4, pp.915-929, 2000.

Y. Wang, J. Yang, X. Wu, L. Li, S. Sun et al., Progress of Spectral Probes for Nucleic Acids, Analytical Letters, vol.40, issue.10, pp.2063-2094, 2003.
DOI : 10.1007/s002160051434

R. A. Carduallo, S. Agrawal, C. Flores, P. C. Zamecnik, and D. E. Wolf, Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfert, Proc. Natl. Acad. Sci. USA, pp.8790-8794, 1988.

S. Tyagi and F. R. Kramer, Molecular Beacons: Probes that Fluoresce upon Hybridization, Nature Biotechnology, vol.13, issue.3, pp.303-308, 1996.
DOI : 10.1073/pnas.93.18.9881

K. Wiederholt, S. B. Rajur, J. Giuliano, M. J. O-'donnell, and L. W. Mclaughin, DNA-Tethered Hoechst Groove-Binding Agents:?? Duplex Stabilization and Fluorescence Characteristics, Journal of the American Chemical Society, vol.118, issue.30, pp.7055-7062, 1995.
DOI : 10.1021/ja960948m

C. Dueymes, J. Décout, P. Peltié, and M. Fontecave, Fluorescent Deazaflavin-Oligonucleotide Probes for Selective Detection of DNA, Angewandte Chemie International Edition, vol.83, issue.3, pp.486-489, 2002.
DOI : 10.1002/1521-3773(20020201)41:3<486::AID-ANIE486>3.0.CO;2-O

G. N. Grimm, A. S. Boutorine, and P. Lincoln, Formation of DNA Triple Helices by an Oligonucleotide Conjugated to a Fluorescent Ruthenium Complex, ChemBioChem, vol.121, issue.4, pp.324-331, 2002.
DOI : 10.1002/1439-7633(20020402)3:4<324::AID-CBIC324>3.0.CO;2-7

K. Yamana, H. Zako, K. Asazuma, R. Iwase, H. Nakano et al., Fluorescence Detection of Specific RNA Sequences Using 2???-Pyrene-Modified Oligoribonucleotides, Angewandte Chemie International Edition, vol.42, issue.6, pp.1104-1106, 2001.
DOI : 10.1002/1521-3773(20010316)40:6<1104::AID-ANIE11040>3.0.CO;2-2

N. T. Thuong and M. Chassignol, Synthese et reactivite d'oligothymidylates substitues par un agent intercalant et un groupe thiophosphate, Tetrahedron Letters, vol.28, issue.36, pp.4157-4160, 1987.
DOI : 10.1016/S0040-4039(00)95565-5

J. E. Celebuski and C. Chan, Synthesis and utility of a DNA phosphorylating agent based on 2-(triphenylsilyl)ethanol, The Journal of Organic Chemistry, vol.57, issue.20, pp.5535-5538, 1992.
DOI : 10.1021/jo00046a044

A. Guzaev, H. Salo, A. Azhayev, and H. Lönnberg, A new approach for chemical phosphorylation of oligonucleotides at the 5???-terminus, Tetrahedron, vol.51, issue.34, pp.9375-9384, 1995.
DOI : 10.1016/0040-4020(95)00544-I

M. Leuck, K. E. Vagle, J. S. Roach, and A. Wolter, A novel reagent for the chemical phosphorylation of oligonucleotides, Tetrahedron Letters, vol.45, issue.2, pp.321-324, 2004.
DOI : 10.1016/j.tetlet.2003.10.167

J. E. Marugg, M. Tromp, E. Kuyl-yeheskiely, G. A. Van-der-marel, J. H. Van-boom et al., ChemInform Abstract: A Convenient and General Approach to the Synthesis of Properly Protected d-Nucleoside-3???-hydrogenphosphonates via Phosphite Intermediates., Chemischer Informationsdienst, vol.27, issue.47, pp.2661-2664, 1986.
DOI : 10.1002/chin.198647363

R. Lartia and U. Asseline, New reagent for the preparation of oligonucleotides involving a 5???-thiophosphate or a 5???-phosphate group, Tetrahedron Letters, vol.45, issue.31, pp.5949-5952, 2004.
DOI : 10.1016/j.tetlet.2004.06.067

J. P. Danehy and W. E. Hunter, Alkaline decomposition of organic disulfides. II. Alternative pathways as determined by structure, The Journal of Organic Chemistry, vol.32, issue.7, pp.2047-2053, 1967.
DOI : 10.1021/jo01282a001

R. H. Alul, C. N. Singman, G. Zhang, and R. L. Letsinger, Oxalyl-CPG: a labile support for synthesis of sensitive oligonucleotide derivatives, Nucleic Acids Research, vol.19, issue.7, pp.1527-1532, 1991.
DOI : 10.1093/nar/19.7.1527

O. Hydroquinone-o, -diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis

R. T. Pon, S. Yu, and N. Ac, as determined by [69]: Solid-phase synthesis of modified oligodeoxyribonucleotides with an acridine derivative or a thiophosphate group at their 3?end, Res. Asseline, U. Tetrahedron Lett, vol.25, issue.30, pp.3629-3635, 1989.

E. H. Rodd, A. Mishra, R. K. Behera, P. K. Behera, B. K. Mishra et al., Cyanines during the 1990's: A review, Chem. Rev, vol.72, issue.100, pp.1973-2011, 2000.

M. Nissum, J. P. Jacobsen, O. Nielsen, W. Jensen, and P. , Determination of the stability of complexes between DNA and the thiazole orange derivatives TO6 and TOTO by surface-enhanced resonance Raman spectroscopy, Biospectroscopy, vol.34, issue.3, pp.207-213, 1997.
DOI : 10.1002/(SICI)1520-6343(1997)3:3<207::AID-BSPY4>3.0.CO;2-2

L. G. Brooker, G. H. Keyes, and W. W. Williams, The Absorption of Unsymmetrical Cyanines. Resonance as a Basis for a Classification of Dyes, Journal of the American Chemical Society, vol.64, issue.2, pp.199-210, 1942.
DOI : 10.1021/ja01254a002

T. G. Deligeorgiev, N. I. Gadjev, K. H. Drexhage, and R. W. Sabnis, Preparation of intercalating dye thiazole orange and derivatives, Dyes and Pigments, vol.29, issue.4, pp.315-322, 1995.
DOI : 10.1016/0143-7208(95)00056-9

T. D. Deligeorgiev, D. A. Zaneva, H. E. Katerinopoulos, and V. N. Kolev, A novel method for the preparation of monomethine cyanine dyes, Dyes and Pigments, vol.41, issue.1-2, pp.49-54, 1999.
DOI : 10.1016/S0143-7208(98)00061-8

T. D. Deligeorgiev, D. A. Zaneva, S. H. Kim, and R. W. Sabnis, Preparation of monomethine cyanine dyes for nucleic acid detection, Dyes and Pigments, vol.37, issue.3, pp.205-211, 1998.
DOI : 10.1016/S0143-7208(97)80088-5

J. Isacsson and G. Westman, Solid-phase synthesis of asymmetric cyanine dyes, Tetrahedron Letters, vol.42, issue.18, pp.3207-3210, 2001.
DOI : 10.1016/S0040-4039(01)00365-3

J. Sandstrom, G. Westmann, and U. Brevet, Probe for analysis of nucleic acids, 2004.

H. S. Rye, S. Yue, D. E. Wemmer, M. A. Quesada, R. P. Haugland et al., Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications, Nucleic Acids Research, vol.20, issue.11, pp.2803-2812, 1992.
DOI : 10.1093/nar/20.11.2803

S. Prodhomme, J. Demaret, S. Vinogradov, U. Asseline, L. Morin-allory et al., A theoretical and experimental study of two thiazole orange derivatives with single- and double-stranded oligonucleotides, polydeoxyribonucleotides and DNA, Journal of Photochemistry and Photobiology B: Biology, vol.53, issue.1-3, pp.60-69, 1999.
DOI : 10.1016/S1011-1344(99)00127-X

L. G. Lee, C. Chen, and L. A. Liu, Thiazole orange: A new dye for reticulocyte analysis, Cytometry, vol.61, issue.6, pp.508-517, 1986.
DOI : 10.1002/cyto.990070603

T. L. Netzel, K. Nafisi, M. Zhao, J. R. Lenhard, and I. Johnson, Base-Content Dependence of Emission Enhancements, Quantum Yields, and Lifetimes for Cyanine Dyes Bound to Double-Strand DNA: Photophysical Properties of Monomeric and Bichromomphoric DNA Stains, The Journal of Physical Chemistry, vol.99, issue.51, pp.17936-17947, 1995.
DOI : 10.1021/j100051a019

J. T. Petty, J. A. Bordelon, and M. E. Robertson, Thermodynamic Characterization of the Association of Cyanine Dyes with DNA, The Journal of Physical Chemistry B, vol.104, issue.30, pp.7221-7227, 2000.
DOI : 10.1021/jp000916s

D. L. Boger and W. C. Tse, Thiazole orange as the fluorescent intercalator in a high resolution fid assay for determining DNA binding affinity and sequence selectivity of small molecules, Bioorganic & Medicinal Chemistry, vol.9, issue.9, pp.2511-2518, 2001.
DOI : 10.1016/S0968-0896(01)00243-7

A. Larsson, C. Carlsson, M. Jonsson, and B. Albinsson, Characterization of the Binding of the Fluorescent Dyes YO and YOYO to DNA by Polarized Light Spectroscopy, Journal of the American Chemical Society, vol.116, issue.19, pp.8459-8465, 1994.
DOI : 10.1021/ja00098a004

Y. , Y. Carlsson, C. Larsson, A. Jonsson, M. Albinsson et al., Optical and photophysical properties of the oxazole yellow DNA probes, J.Phys.Chem, vol.98, pp.10313-10321, 1994.

B. Åkermann and E. Tuite, Single- and double-strand photocleavage of DNA by YO, YOYO and TOTO, Nucleic Acids Research, vol.24, issue.6, pp.1080-1090, 1996.
DOI : 10.1093/nar/24.6.1080

B. Gaugain, J. Barbet, R. Oberlin, B. P. Roques, L. Pecq et al., DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer, Biochemistry, vol.17, issue.24, pp.5071-5078, 1978.
DOI : 10.1021/bi00617a001

S. C. Benson, R. A. Mathies, and A. N. Glazer, Heterodimeric DNA-binding dyes designed for energy transfer: stability and applications of the DNA complexes, Nucleic Acids Research, vol.21, issue.24, pp.5720-5726, 1993.
DOI : 10.1093/nar/21.24.5720

H. P. Spielmann, D. E. Wemmer, and J. P. Jacobsen, Solution Structure of a DNA Complex with the Fluorescent Bis-Intercalator TOTO Determined by NMR Spectroscopy, Biochemistry, vol.34, issue.27, pp.8542-8553, 1995.
DOI : 10.1021/bi00027a004

K. Bondensgaard and J. P. Jacobsen, Solution Structure and Energy Calculation of Bis-Intercalation of Homodimeric Thiazole Orange Dye Derivatives in DNA:?? Effects of Modifying the Linker, Bioconjugate Chemistry, vol.10, issue.5, pp.735-744, 1999.
DOI : 10.1021/bc9900284

M. Petersen and J. P. Jacobsen, Solution Structure of a DNA Complex with the Fluorescent Bis-Intercalator TOTO Modified on the Benzothiazole Ring, Bioconjugate Chemistry, vol.9, issue.3, pp.331-340, 1998.
DOI : 10.1021/bc970133i

L. K. Jensen, C. H. Gotfredsen, K. Bondensgaard, and J. P. Jacobsen, 641- 650. g sites of [100]: Bis-intercalation of a homodimeric thiazole orange dye in DNA in symmetrical pyrimidinepyrimidine-purine-purine oligonucleotides, Acta. Chem. Scand, vol.52, 1998.

L. F. Hansen, L. K. Jensen, and J. P. Jacobsen, Bis-Intercalation of a Homodimeric Thiazole Orange Dye in DNA in Symmetrical Pyrimidine-Pyrimidine-Purine-Purine Oligonucleotides, Nucleic Acids Research, vol.24, issue.5, pp.859-867, 1996.
DOI : 10.1093/nar/24.5.859

J. P. Jacobsen, J. B. Pedersen, L. F. Hansen, and D. E. Wemmer, Site selective bis-intercalation of a homodimeric thiazole orange dye in DNA oligonucleotides, Nucleic Acids Research, vol.23, issue.5, pp.753-760, 1995.
DOI : 10.1093/nar/23.5.753

J. Bunkenborg, N. I. Gadjev, T. Deligeorgiev, and J. P. Jacobsen, Concerted Intercalation and Minor Groove Recognition of DNA by a Homodimeric Thiazole Orange Dye, Bioconjugate Chemistry, vol.11, issue.6, pp.861-867, 2000.
DOI : 10.1021/bc000042c

Z. Zeng, S. C. Benson, and A. N. Glazer, Fluorescence Energy-Transfer Cyanine Heterodimers with High Affinity for Double-Stranded DNA II. Applications to Multiplex Restriction Fragment Sizing, Analytical Biochemistry, vol.231, issue.1, pp.256-260, 1995.
DOI : 10.1006/abio.1995.1528

C. Carlsson, M. Jonsson, and B. Åkerman, Double bands in DNA gel elctrophoresis caused by bis-intercalating dyes, Nucleic Acids Research, vol.23, issue.13, pp.2413-2420, 1995.
DOI : 10.1093/nar/23.13.2413

H. S. Rye and A. N. Glazer, Interaction of dimeric intercalating dyes with single-stranded DNA, Nucleic Acids Research, vol.23, issue.7, pp.1215-1222, 1995.
DOI : 10.1093/nar/23.7.1215

L. G. Lee, Biometric Imaging Inc.) Brevet US 5,734,058. 31 mars, 1998.

I. A. Struganova, M. Hazell, J. Gaitor, D. Mcnally-carr, and S. Zivanovic, Influence of Inorganic Salts and Bases on the J-Band in the Absorption Spectra of Water Solutions of 1,1???-Diethyl-2,2???-cyanine Iodide, The Journal of Physical Chemistry A, vol.107, issue.15, pp.2650-2656, 2003.
DOI : 10.1021/jp0223004

N. I. Gadjev, T. G. Deligeorgiev, and S. H. Kim, Preparation of monomethine cyanine dyes as noncovalent labels for nucleic acids, Dyes and Pigments, vol.40, issue.2-3, pp.181-186, 1999.
DOI : 10.1016/S0143-7208(98)00046-1

W. H. Mills and W. T. Braunholtz, CLXXV.???The cyanine dyes. Part V. The virtual tautomerism of the thiocyanines, J. Chem. Soc., Trans., vol.121, issue.0, pp.1489-1495, 1922.
DOI : 10.1039/CT9222101489

S. M. Yarmoluk, S. S. Lukashov, T. Y. Ogul-'chansky, M. Y. Losytsky, and O. S. Kornyushyna, Interaction of cyanine dyes with nucleic acids. XXI. Arguments for half-intercalation model of interaction, Biopolymers, vol.56, issue.4, pp.219-227, 2001.
DOI : 10.1002/bip.1016

T. Y. Losytskyy, M. Y. Kovalska, V. B. Yashchuk, V. M. Yarmoluk, and S. M. , Interaction of cyanine dyes with nucleic acids XXIV : Aggregation of monomethine cyanine dyes in presence of DNA and its manifestation in absorption and fluorescence spectra, Spectrochim. Acta. A, vol.117, issue.57, pp.1525-1532, 2001.

M. R. Dye-sahyun, J. T. Blair, and J. , Photophysics of a ???simple??? cyanide dye, Journal of Photochemistry and Photobiology A: Chemistry, vol.104, issue.1-3, pp.179-187, 1997.
DOI : 10.1016/S1010-6030(96)04481-4

K. N. Ganesh and P. E. Nielsen, Peptide Nucleic Acids: Analogs and Derivatives, Current Organic Chemistry, vol.4, issue.9, pp.931-943, 2000.
DOI : 10.2174/1385272003375969

N. Svanvik, J. Nygren, G. Westman, and M. Kubista, Free-Probe Fluorescence of Light-up Probes, Journal of the American Chemical Society, vol.123, issue.5, pp.803-809, 2001.
DOI : 10.1021/ja002294u

N. Svanvik, G. Westmann, D. Wang, and M. Kubista, Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution, Analytical Biochemistry, vol.281, issue.1, pp.26-35, 2000.
DOI : 10.1006/abio.2000.4534

E. Privat, T. Melvin, U. Asseline, and P. Vigny, Oligonucleotide-conjugated Thiazole Orange Probes as ???Light-up??? Probes for Messenger Ribonucleic Acid Molecules in Living Cells??, Photochemistry and Photobiology, vol.290, issue.4, pp.532-540, 2001.
DOI : 10.1562/0031-8655(2001)074<0532:OCTOPA>2.0.CO;2

E. Privat, T. Melvin, F. Mérola, G. Schweizer, S. Prodhomme et al., Fluorescent Properties of Oligonucleotide-conjugated Thiazole Orange Probes??, Photochemistry and Photobiology, vol.98, issue.3, pp.201-210, 2002.
DOI : 10.1562/0031-8655(2002)075<0201:FPOOCT>2.0.CO;2

O. Seitz, F. Bergmann, and D. Heindl, A Convergent Strategy for the Modification of Peptide Nucleic Acids: Novel Mismatch-Specific PNA-Hybridization Probes, Angewandte Chemie International Edition, vol.38, issue.15, pp.2203-2206, 1999.
DOI : 10.1002/(SICI)1521-3773(19990802)38:15<2203::AID-ANIE2203>3.0.CO;2-2

E. Privat and U. Asseline, Synthesis and Binding Properties of Oligo-2???-deoxyribonucleotides Covalently Linked to a Thiazole Orange Derivative, Bioconjugate Chemistry, vol.12, issue.5, pp.757-769, 2001.
DOI : 10.1021/bc0100051

R. Raghavachari and W. Tan, Edition, Genomics & Proteomics technologies Proceeding of SPIE International Society of Optical Engineering, vol.4264, pp.53-58, 2001.

N. Svanvik, A. Ståhlberg, U. Sehlstedt, R. Sjöback, and M. Kubista, Detection of PCR Products in Real Time Using Light-up Probes, Analytical Biochemistry, vol.287, issue.1, pp.179-182, 2000.
DOI : 10.1006/abio.2000.4824

A. Ståhlberg, P. Åman, B. Ridell, P. Mostad, and M. Kubista, Quantitative Real-Time PCR Method for Detection of B-Lymphocyte Monoclonality by Comparison of ?? and ?? Immunoglobulin Light Chain Expression, Clinical Chemistry, vol.49, issue.1, pp.51-59, 2003.
DOI : 10.1373/49.1.51

J. B. Tok, J. Cho, and R. R. Rando, Aminoglycoside hybrids as potent RNA antagonists, Tetrahedron, vol.55, issue.18, pp.5741-5758, 1999.
DOI : 10.1016/S0040-4020(99)00240-9

J. Babendure, P. A. Liddell, R. Bash, D. Lovullo, T. K. Schiefer et al., Development of a fluorescent probe for the study of nucleosome assembly and dynamics, Analytical Biochemistry, vol.317, issue.1, pp.1-11, 2003.
DOI : 10.1016/S0003-2697(03)00085-X

J. R. Carreon, K. P. Mahon, and S. O. Kelley, Thiazole Orange???Peptide Conjugates:??? Sensitivity of DNA Binding to Chemical Structure, Organic Letters, vol.6, issue.4, pp.517-519, 2004.
DOI : 10.1021/ol0362818

X. Wang and U. J. Krull, Tethered thiazole orange intercalating dye for development of fibre-optic nucleic acid biosensors, Analytica Chimica Acta, vol.470, issue.1, pp.57-70, 2002.
DOI : 10.1016/S0003-2670(02)00671-2

T. Ishiguro, J. Saitoh, H. Yawata, M. Otsuka, T. Inoue et al., Fluorescence Detection of Specific Sequence of Nucleic Acids by Oxazole Yellow-Linked Oligonucleotides. Homogeneous Quantitative Monitoring of In Vitro Transcription, Nucleic Acids Research, vol.24, issue.24, pp.4992-4997, 1996.
DOI : 10.1093/nar/24.24.4992

T. Inoue, Y. Sugiura, J. Saitoh, T. Ishiguro, and M. Otsuka, Fluorescence property of oxazole yellow-linked oligonucleotide. Triple helix formation and photocleavage of double-stranded DNA in the presence of spermine, Bioorganic & Medicinal Chemistry, vol.7, issue.6, pp.1207-1211, 1999.
DOI : 10.1016/S0968-0896(99)00035-8

U. Asseline, E. Bonfils, D. Dupret, and N. T. Thuong, Synthesis and Binding Properties of Oligonucleotides Covalently Linked to an Acridine Derivative:?? New Study of the Influence of the Dye Attachment Site, Bioconjugate Chemistry, vol.7, issue.3, pp.369-379, 1996.
DOI : 10.1021/bc960024o

W. W. Davis, M. E. Krahl, and G. H. Clowes, Solubility of Carcinogenic and Related Hydrocarbons in Water, Journal of the American Chemical Society, vol.64, issue.1, pp.108-110, 1942.
DOI : 10.1021/ja01253a028

B. Green and J. A. Mccarter, Polarized fluorescence of polycyclic hydrocarbons in aqueous DNA solutions: Effect of flow-orientation, Journal of Molecular Biology, vol.29, issue.3, pp.447-456, 1967.
DOI : 10.1016/0022-2836(67)90111-8

C. Nagata, M. Kodama, Y. Tagashira, and A. Imamura, Interaction of polynuclear aromatic hydrocarbons, 4-nitroquinoline 1-oxides, and various dyes with DNA, Biopolymers, vol.66, issue.4, pp.409-427, 1966.
DOI : 10.1002/bip.1966.360040403

A. Wolfe, G. H. Shimer, and T. Meehan, Polycyclic aromatic hydrocarbons physically intercalate into duplex regions of denatured DNA, Biochemistry, vol.26, issue.20, pp.6392-6396, 1987.
DOI : 10.1021/bi00394a013

F. Chen, Binding of pyrene to DNA, base squence specificity and its implication, Nucleic Acids Research, vol.11, issue.20, pp.7231-7251, 1983.
DOI : 10.1093/nar/11.20.7231

N. E. Geacintov, T. Prusik, and J. M. Khosrofian, Properties of benzopyrene-DNA complexes investigated by fluorescence and triplet flash photolysis techniques, Journal of the American Chemical Society, vol.98, issue.21, pp.6444-6452, 1976.
DOI : 10.1021/ja00437a002

D. Schryver, F. C. Collart, P. Vandendriessche, J. Goedeweeck, R. Swinnen et al., Intramolecular excimer formation in bichromophoric molecules linked by a short flexible chain, Accounts of Chemical Research, vol.20, issue.5, pp.159-166, 1987.
DOI : 10.1021/ar00137a001

F. D. Lewis, Y. Zhang, and R. L. Letsinger, Bispyrenyl Excimer Fluorescence:?? A Sensitive Oligonucleotide Probe, Journal of the American Chemical Society, vol.119, issue.23, pp.5451-5452, 1997.
DOI : 10.1021/ja9641214

L. P. Paris, J. M. Langenhan, and E. T. Kool, Probing DNA sequences in solution with a monomer-excimer fluorescence color change, Nucleic Acids Research, vol.26, issue.16, pp.3789-3793, 1998.
DOI : 10.1093/nar/26.16.3789

K. Ebata, M. Masuko, H. Ohtani, and M. Kashiwasake-jibu, NUCLEIC ACID HYBRIDIZATION ACCOMPANIED WITH EXCIMER FORMATION FROM TWO PYRENE-LABELED PROBES, Photochemistry and Photobiology, vol.143, issue.5, pp.836-839, 1995.
DOI : 10.1111/j.1751-1097.1995.tb09144.x

M. Masuko, H. Ohtani, K. Ebata, and A. Shimadzu, Optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore, Nucleic Acids Research, vol.26, issue.23, pp.5409-5416, 1998.
DOI : 10.1093/nar/26.23.5409

K. Yamana, M. Takei, and H. Nakano, Synthesis of oligonucleotide derivatives containing pyrene labeled glycerol linkers: Enhhanced excimer fluorescence on binding to a complementary DNA sequence, Tetrahedron Letters, vol.38, issue.34, pp.6051-6054, 1997.
DOI : 10.1016/S0040-4039(97)01358-0

U. B. Christensen and E. B. Pedersen, Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA, Nucleic Acids Research, vol.30, issue.22, pp.4918-4925, 2002.
DOI : 10.1093/nar/gkf624

A. Okamoto, T. Ichiba, and I. Saito, Pyrene-Labeled Oligodeoxynucleotide Probe for Detecting Base Insertion by Excimer Fluorescence Emission, Journal of the American Chemical Society, vol.126, issue.27, pp.8364-8365, 2004.
DOI : 10.1021/ja049061d

K. Yamana, T. Iwai, Y. Ohtani, S. Sato, M. Nakamura et al., Bis-Pyrene-Labeled Oligonucleotides:?? Sequence Specificity of Excimer and Monomer Fluorescence Changes upon Hybridization with DNA, Bioconjugate Chemistry, vol.13, issue.6, pp.1266-1273, 2002.
DOI : 10.1021/bc025530u

A. Okamoto, K. Kanatani, Y. Ochi, Y. Saito, and I. Saito, A novel fluorescent guanine derivative distinguishable of three structures, single strand, duplex, and quadruplex, Tetrahedron Letters, vol.45, issue.31, pp.6059-6062, 2004.
DOI : 10.1016/j.tetlet.2004.05.136

J. D. Frazer, S. M. Horner, and S. A. Woski, Synthesis of a novel pyrene-containing nucleoside and its incorporation into oligonucleotides, Tetrahedron Letters, vol.39, issue.11, pp.1279-1282, 1998.
DOI : 10.1016/S0040-4039(97)10853-X

N. N. Dioubankova, A. D. Malakhov, D. A. Stetsenko, M. J. Gait, P. E. Volynsky et al., Pyrenemethyl ara-Uridine-2???-carbamate: A Strong Interstrand Excimer in the Major Groove of a DNA Duplex, ChemBioChem, vol.4, issue.9, pp.841-847, 2003.
DOI : 10.1002/cbic.200300678

G. Tong, J. M. Lawlor, G. W. Tregear, and J. Haralambidis, Oligonucleotide-Polyamide Hybrid Molecules Containing Multiple Pyrene Residues Exhibit Significant Excimer Fluorescence, Journal of the American Chemical Society, vol.117, issue.49, pp.12151-12158, 1995.
DOI : 10.1021/ja00154a015

M. Craig and I. Isenberg, Testing of A size criterion for DNA-hydrocarbon binding, Biopolymers, vol.18, issue.6, pp.689-696, 1970.
DOI : 10.1002/bip.1970.360090607

K. W. Bair, C. W. Andrews, R. L. Tuttle, V. C. Knick, M. Cory et al., 2-[(Arylmethyl)amino]-2-methyl-1,3-propanediol DNA intercalators. An examination of the effects of aromatic ring variation on antitumor activity and DNA binding, Journal of Medicinal Chemistry, vol.34, issue.7, pp.1983-1990, 1991.
DOI : 10.1021/jm00111a010

L. Jinshui, W. Lun, G. Feng, L. Yongxing, and W. Yun, Novel fluorescent colloids as a DNA fluorescence probe, Analytical and Bioanalytical Chemistry, vol.377, issue.2, pp.346-349, 2003.
DOI : 10.1007/s00216-003-2098-4

K. V. Balakin, V. A. Korshun, I. I. Mikhalev, G. V. Maleev, A. D. Malakhov et al., Erratum to: ???Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes???, Erratum: Biosensors and Bioelectronics, pp.771-778, 1998.
DOI : 10.1016/S0956-5663(99)00033-0

K. V. Balakin, V. A. Korshun, D. S. Esipov, I. I. Mikhalev, and Y. A. Berlin, Perylene-Labeled Oligonucleotide as a Probe in Homogeneous Hybridization Assay, Nucleosides and Nucleotides, vol.5, issue.6-7, pp.1279-1280, 1999.
DOI : 10.1080/07328319908044691

V. V. Koval, D. V. Pyshnyi, and O. S. Fedorova, CD and Melting Curves Structural Studies of the Tandem DNA Complex Formed with Oligonucleotides Carrying Photoactive and Sensitizing Groups in the Nick Region, Journal of Biomolecular Structure and Dynamics, vol.17, issue.3, pp.515-526, 2001.
DOI : 10.1007/BF00128336

J. Gao, C. Strässler, D. Tahmassebi, and E. T. Kool, Libraries of Composite Polyfluors Built from Fluorescent Deoxyribosides, Journal of the American Chemical Society, vol.124, issue.39, pp.11590-11591, 2002.
DOI : 10.1021/ja027197a

Y. Aubert and U. Asseline, Nucleoside Nucleotide Nucleic Acids, pp.1223-1225, 2003.

S. Bevers, T. P. O-'dea, and L. W. Mclaughin, Perylene- and Naphthalene-Based Linkers for Duplex and Triplex Stabilization, Journal of the American Chemical Society, vol.120, issue.42, pp.11004-11005, 1998.
DOI : 10.1021/ja981962x

S. Bevers, S. Schutte, and L. W. Mclaughin, Naphthalene- and Perylene-Based Linkers for the Stabilization of Hairpin Triplexes, Journal of the American Chemical Society, vol.122, issue.25, pp.5905-5915, 2000.
DOI : 10.1021/ja0001714

W. Wang, W. Wei, H. Zhou, S. Niu, and A. D. Li, Alternating DNA and ??-Conjugated Sequences. Thermophilic Foldable Polymers, Journal of the American Chemical Society, vol.125, issue.18, pp.5248-5249, 2003.
DOI : 10.1021/ja0341900

W. Tuntiwechapikul, T. J. Lee, and M. Salazar, Design and Synthesis of the G-Quadruplex-Specific Cleaving Reagent Perylene-EDTA??Iron(II), Journal of the American Chemical Society, vol.123, issue.23, pp.5606-5607, 2001.
DOI : 10.1021/ja0156439

L. Rossetti, M. Franceshin, A. Bianco, G. Ortaggi, and M. Savino, Perylene diimides with different side chains are selective in inducing different G-Quadruplex DNA structures and in inhibiting telomerase, Bioorganic & Medicinal Chemistry Letters, vol.12, issue.18, pp.2527-2533, 2002.
DOI : 10.1016/S0960-894X(02)00504-8

J. T. Kern and S. M. Kerwin, The aggregation and G-quadruplex DNA selectivity of charged 3,4,9,10-perylenetetracarboxylic acid diimides, Bioorganic & Medicinal Chemistry Letters, vol.12, issue.23, pp.3395-3398, 2002.
DOI : 10.1016/S0960-894X(02)00763-1

M. S. Shehepinov and V. A. Korshun, Design of multidye systems for FRET-based applications, Nucleoside Nucleotide Nucleic Acids, vol.20, pp.4-7, 2001.

A. Yamane, MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator, Nucleic Acids Research, vol.30, issue.19, p.97, 2002.
DOI : 10.1093/nar/gnf096

A. Zinke, H. Troger, and E. Ziegler, Untersuchungen ??ber Perylen und seine Derivate, L. Mitteilung, Berichte der deutschen chemischen Gesellschaft (A and B Series), vol.68, issue.II, pp.1042-1048, 1940.
DOI : 10.1002/cber.19400731003

L. B. Johansson, -. Molotovsky, J. G. Bergelson, and L. D. , Fluorescence and absorption properties of perylenyl and perylenoyl probe molecules in solvents and liquid crystals, Journal of the American Chemical Society, vol.109, issue.24, pp.7374-7387, 1987.
DOI : 10.1021/ja00258a021

R. H. Mitchell, Y. Lai, and R. V. Williams, N-Bromosuccinimide-dimethylformamide: a mild, selective nuclear monobromination reagent for reactive aromatic compounds, The Journal of Organic Chemistry, vol.44, issue.25, pp.4733-4735, 1979.
DOI : 10.1021/jo00393a066

S. Hung, J. Ju, R. A. Mathies, and A. N. Glazer, Cyanine Dyes with High Absorption Cross Section as Donor Chromophores in Energy Transfer Primers1, Analytical Biochemistry, vol.243, issue.1, pp.15-27, 1996.
DOI : 10.1006/abio.1996.0477

O. Baudoin, M. Teulade-fichou, J. Vigneron, and J. Lehn, Cyclobisintercaland Macrocycles:?? Synthesis and Physicochemical Properties of Macrocyclic Polyamines Containing Two Crescent-Shaped Dibenzophenanthroline Subunits, The Journal of Organic Chemistry, vol.62, issue.16, pp.5458-5470, 1997.
DOI : 10.1021/jo970496b

M. Manoharan, C. J. Guinosso, and P. D. Cook, Novel functionalization of the sugar moiety of nucleic acids for multiple labeling in the minor groove, Tetrahedron Letters, vol.32, issue.49, pp.7171-7174, 1991.
DOI : 10.1016/0040-4039(91)80468-L

J. B. Birks and L. G. Christophorou, 'Excimer' Fluorescence. IV. Solution Spectra of Polycyclic Hydrocarbons, Proc. Roy. Soc, pp.571-582, 1964.
DOI : 10.1098/rspa.1964.0041

R. Katoh, E. Katoh, N. Nakashima, M. Yuuki, and M. Kotani, Near-IR Absorption Spectrum of Aromatic Excimers, The Journal of Physical Chemistry A, vol.101, issue.42, pp.7725-7728, 1997.
DOI : 10.1021/jp964075u