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CONTRIBUTION A L'ETUDE STRUCTURALE ET FONCTIONNELLE DU FACTEUR DE TRANSCRIPTION TFIIH

Abstract : TFIIH is a general transcription factor involved in class II gene transcription as well as nucleotide excision repair. In human, mutations in both helicases of TFIIH, XPD and XPB, are directly incriminated in three diseases: the Xeroderma Pigmentosum, the Cockayne Syndrome and trichothiodystrophie. During my PhD studies, I have been interested in the structure and function relationships of the TFIIH human factor. We have characterized the recombinant TFIIH complex in electronic microscopic and its structure was identical to the endogenous one. We have used this structure to dock the biochemical informations obtain by protein-protein interaction studies and we propose a general model of the molecular organization of the human TFIIH. Finally a study aimed to put TFIIH with natural substrates, DNA or partners, was done. I was implicated in the development of a new technology designed to immobilize DNA on a solid support suitable for electron microscopy observation. We also reconstruct a cryo-negative staining model of TFIIE, which interacts physically with TFIIH, at 1.6nm resolution. Finally, we obtain 3D crystals of the homologue of XPD helicase in an archaebacteria
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https://tel.archives-ouvertes.fr/tel-00009083
Contributor : Muriel Uhring <>
Submitted on : Monday, April 25, 2005 - 11:07:49 AM
Last modification on : Friday, October 23, 2020 - 4:38:21 PM
Long-term archiving on: : Friday, November 25, 2016 - 9:06:55 AM

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  • HAL Id : tel-00009083, version 1

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Muriel Uhring. CONTRIBUTION A L'ETUDE STRUCTURALE ET FONCTIONNELLE DU FACTEUR DE TRANSCRIPTION TFIIH. Biochimie [q-bio.BM]. Université Louis Pasteur - Strasbourg I, 2004. Français. ⟨tel-00009083⟩

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