Skip to Main content Skip to Navigation
Theses

Etude de la régulation transcriptionnelle des gènes lors du cycle érythrocytaire de Plasmodium falciparum

Abstract : During the life cycle of Plasmodium falciparum, the events occurring during the erythrocytic cycle implies a fine control of transcriptional regulation. We examined the regulations which may be involved in key events of the parasite life cycle. We initiated the analysis of gene expression during the erythrocytic development of two clones of P. falciparum, 3D7 and F12 (non-gametocyte producer). Using a thematic DNA microarray, we identify genes bearing differential expression profiles. They may participate in the transition towards sexual differentiation. We showed that a transcription factor, PfMyb1, has a specific DNA binding activity. We tried to understand its role during the erythrocytic cycle. Culture treated with pfmyb1 dsRNA exhibited a growth inhibition. We investigated the effect of the partial loss of availability of the transcript using the thematic DNA microarray. We worked on the promoters, in order to understand factors determining gene regulation by PfMyb1.
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-00008492
Contributor : Mathieu Gissot <>
Submitted on : Monday, February 14, 2005 - 7:12:41 PM
Last modification on : Tuesday, December 8, 2020 - 3:36:35 AM
Long-term archiving on: : Friday, November 25, 2016 - 8:54:13 AM

Identifiers

  • HAL Id : tel-00008492, version 1

Citation

Mathieu Gissot. Etude de la régulation transcriptionnelle des gènes lors du cycle érythrocytaire de Plasmodium falciparum. Biochimie [q-bio.BM]. Université Pierre et Marie Curie - Paris VI, 2005. Français. ⟨tel-00008492⟩

Share

Metrics

Record views

368

Files downloads

6345