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Propriétés optiques de marqueurs fluorescents d'intérêt biologique en interaction avec leur environnement : étude par spectroscopie femtoseconde

Abstract : During this experimental work, we have investigated the photo-physical properties of a Green Fluorescent Protein mutant (Green Fluorescent Protein, GFP). In particular, we have characterized the excited-states dynamics of this mutant by using spectrally time-resolved femtosecond spectroscopy. The comparison between the results obtained for the mutant and those obtained for the wild type protein allowed us to show that the excited- states dynamics is very sensitive to the modifications of the local environment of the optically active part of the protein. In a second part, devoted to the study of genetic fusion antibody fragment-GFP, we have used this sensitivity to characterize the final folded state of the antibody fragment. In another part of this work we have studied the dynamical properties of some coumarin derivatives whose molecular rotors character has been evoked in the literature. This study showed that the excited-states dynamics do not present signatures of such a behavior. Finally, we have characterized the aging mechanism or the reduction in the number of fluorescent molecules of a well-defined ensemble of GFP under cw excitation. This study evidenced that the temporal evolution of the system is governed by Lévy statistics. One of the main consequences is the existence of an unstable state with an infinite mean lifetime.
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https://tel.archives-ouvertes.fr/tel-00008127
Contributor : Pascal Didier <>
Submitted on : Wednesday, January 19, 2005 - 4:40:57 PM
Last modification on : Thursday, May 28, 2020 - 3:30:31 AM
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Pascal Didier. Propriétés optiques de marqueurs fluorescents d'intérêt biologique en interaction avec leur environnement : étude par spectroscopie femtoseconde. Physique Atomique [physics.atom-ph]. Université Louis Pasteur - Strasbourg I, 2004. Français. ⟨tel-00008127⟩

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