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Diversité structurale et d'activité biologique des Albumines entomotoxiques de type 1b des graines de Légumineuses

Abstract : PA1b (Pea Albumin 1 subunit b), a 37 amino acid cystine-knot toxin, is of great interest for the control of cereal weevils (Sitophilus spp.), major pests of stored cereals.
To know more on the new A1b family, its variability in structure and biological activity was studied. After the validation of our approach on four "test" plant species, we characterised 24 homologous genes from 18 Papilionoideae species. Moreover, the insecticidal activity of seed extracts, from 60 species belonging to the three legumes subfamilies, was determined on weevil strains susceptible and resistant to PA1b. To link structure and activity variations, a mutagenesis approach was proposed. A bacterial expression and purification system for PA1b was determined. However the recombinant peptide was not biologically active, despite harbouring the expected mass (with three oxidised disulfide bridges).
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https://tel.archives-ouvertes.fr/tel-00007903
Contributor : Sandrine Louis <>
Submitted on : Tuesday, January 4, 2005 - 2:10:20 PM
Last modification on : Wednesday, July 8, 2020 - 12:42:09 PM
Long-term archiving on: : Friday, April 2, 2010 - 9:03:55 PM

Identifiers

  • HAL Id : tel-00007903, version 1
  • PRODINRA : 318763

Citation

Sandrine Louis. Diversité structurale et d'activité biologique des Albumines entomotoxiques de type 1b des graines de Légumineuses. Biochimie [q-bio.BM]. INSA de Lyon, 2004. Français. ⟨tel-00007903⟩

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