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Etude par fluorescence de l'importance des structures primaires et secondaires de la séquence cTAR et de la protéine NCp7 lors du premier saut de brin de la transcription inverse de VIH-1

Abstract : NCp7 is implied in many stages of the viral cycle in particular the retrotranscription. This makes NCp7 a potential target for an anti-HIV treatment. By its chaperone activity, it stimulates the first strand-transfer. It consists of the destabilization and hybridization of the TAR and cTAR sequences of the viral genome. It is by using cTAR sequence labelled to their 3' and 5' ends and various techniques of fluorescence spectroscopy and absorption that we could highlight the importance of the primary and secondary structures of NCp7 and cTAR in the first strand-transfer. The destabilizing activity is strongly dependent on the bulges and the internal loop of cTAR. In addition, this activity also depends on the presence of the Trp37 and on the presence of the two zinc fingers in their native form. Then, we could highlight that NCp7 supported the formation of the homodimer of the top half of cTAR by formation of a kissing complex. Finally, we studied a new fluorescent probe analogue to the deoxyadenosine (the 8-vinyl-déoxyadénosine) which is an alternative probe to the 2-aminopurine.
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https://tel.archives-ouvertes.fr/tel-00007730
Contributor : Hervé Beltz <>
Submitted on : Monday, December 13, 2004 - 2:57:37 PM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM
Long-term archiving on: : Thursday, September 13, 2012 - 1:40:36 PM

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  • HAL Id : tel-00007730, version 1

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Hervé Beltz. Etude par fluorescence de l'importance des structures primaires et secondaires de la séquence cTAR et de la protéine NCp7 lors du premier saut de brin de la transcription inverse de VIH-1. Sciences pharmaceutiques. Université Louis Pasteur - Strasbourg I, 2004. Français. ⟨tel-00007730⟩

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