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Développement de biocapteurs électrochimiques à base de tyrosinase pour la détection de polluants organiques en phase aqueuse

Abstract : This work consists of developing two types of microbiosensors; the Ion Sensitive Field Effect Transistor (ISFET) and the conductometers by using enzyme tyrosinase for the detection of pesticides and phenol derivatives in water. These biosensors were fabricated at International Training Institute for Materials Science (ITIMS) - Viet Nam by applying the spin on glass (SOG) technology. The sensors were biofunctionnalized by immobilization of the tyrosinase and the bovin serum albumine (BSA) in presence of glutaraldehyde vapor. The immobilization time, the enzyme loading, the pH, the nature of the substrate, the measure time...were optimized in order to get the best analytical characteristics. These sensors allow detecting, in the aquatic phase, phenolic derivative compounds and pesticides such as diuron and atrazine. The detection limit is of 2,15 ppb for atrazine and 2,33 ppb for diuron, the response time is 1 to 5 minutes. A relative standard deviation of the output signal was estimated at about 5% and a slight drift of 1,5 μS/hour was measured and 90% of the enzyme activity was still maintained after 23 days of storage in buffer solution at 4 °C.
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Contributor : Anh Tuan Mai <>
Submitted on : Wednesday, November 24, 2004 - 5:36:50 PM
Last modification on : Wednesday, November 20, 2019 - 3:21:59 AM
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Anh Tuan Mai. Développement de biocapteurs électrochimiques à base de tyrosinase pour la détection de polluants organiques en phase aqueuse. Matériaux. Université Claude Bernard - Lyon I, 2004. Français. ⟨tel-00007508⟩

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