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DYNAMIQUE DES MEMBRANES HETEROGENES ET EFFETS DES MOLECULES D'ASYMETRIE STERIQUE POSITIVE. ETUDES SUR DES VESICULES GEANTES

Abstract : The model systems (giant unilamellar vesicles, GUVs) represent an essential tool to study the formation, stability, dynamics and the functions of rafts in biological membranes. We developed a methodology for studding domain budding and fission from a heterogeneous GUV. That made it possible: i) to visualize vesicle budding and fission events induced by biological agents (sPLA2, Lyso PC), and, ii) to directly visualize the effects of detergents (Triton X100, Brij 98) on GUVs - the selective solubilization of the Ld/Lo membrane and the budding and fission of Lo domains. These experiments support the idea that DRM extraction from cell membranes was not an artefact. We suggested as well a possible mechanism for the Lo domain vesicle fission induced by molecules having positive steric asymmetry. Finally, our results show that giant heterogeneous vesicles present an essential progress in the development of model membrane systems mimicking biological membrane structure and functions.
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https://tel.archives-ouvertes.fr/tel-00007282
Contributor : Galya Staneva <>
Submitted on : Wednesday, November 3, 2004 - 4:05:49 PM
Last modification on : Friday, December 11, 2020 - 3:29:55 AM
Long-term archiving on: : Monday, September 20, 2010 - 12:10:54 PM

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  • HAL Id : tel-00007282, version 2

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Galya Staneva. DYNAMIQUE DES MEMBRANES HETEROGENES ET EFFETS DES MOLECULES D'ASYMETRIE STERIQUE POSITIVE. ETUDES SUR DES VESICULES GEANTES. Autre [q-bio.OT]. Université Pierre et Marie Curie - Paris VI, 2004. Français. ⟨tel-00007282v2⟩

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