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Nouveaux vecteurs synthétiques fonctionnels pour le transfert de gènes

Abstract : The aim of this thesis was the synthesis and the assessment of new functionals non viral gene delivery systems. Two groups of synthetic vectors were synthesised in order to improve the biodisponibility of non viral vectors and increase specific gene delivery into targeted cells. One group of vectors was based on diblock and random cotelomers combining hydrophilic neutral groups (polyethylene glycol, polyhydroxylated groups) with cationic ones. The other group consisted into polycationic lipids or polymers conjugated to AMD3100 which is a specific ligand of the CXCR4 receptor. These vectors were evaluated as non-specific and specific gene transfer agents on human CXCR4(-) cell lines and CXCR4(+) Jurkat cells. Among these new gene delivery systems, compounds PEG2000-[NH2]n which contains a mean number n of amine functions of 190 exhibited a significant transfection efficiency on A549 cell lines as compared with reference systems. With regard to targeted complexes, compound iBu-[NH2]80-[AMD]4 proved to be, under certain conditions, a specific gene delivery system on Jurkat cells.
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Contributor : Bertrand Le Bon <>
Submitted on : Friday, October 8, 2004 - 3:21:08 PM
Last modification on : Wednesday, October 14, 2020 - 4:23:38 AM
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  • HAL Id : tel-00007070, version 1



Bertrand Le Bon. Nouveaux vecteurs synthétiques fonctionnels pour le transfert de gènes. Autre. Université Nice Sophia Antipolis, 2003. Français. ⟨tel-00007070⟩



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