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Nucléation et croissance de contacts entre cellules biologiques

Abstract : We have studied aggregation of adhesive proteins (cadherins) during cell-cell contact formation. Cadherins are linked to the actin cytoskeleton through a proteic complex. The aim of this thesis was to determine the respective roles for membrane and the actin cytoskeleton : nucleation and growth laws of fluorescent cadherin aggregates were acquired and compared with a theoretical model based on a competition between actin polymerization and membrane tension. We thus supported the structural role of actin which imposes the contact geometry. We also developped a planar adhesion set-up in which a cell expressing fluorescent cadherins adheres on a surface covered with recombinant cadherins. We observed the formation of streak-like adhesive motives by evanescent wave microscopy. As was predicted by physicists, we showed that these aggregates formation was due to an increase in membrane tension.
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https://tel.archives-ouvertes.fr/tel-00006847
Contributor : Hélène Delanoë-Ayari <>
Submitted on : Thursday, September 9, 2004 - 3:33:18 PM
Last modification on : Tuesday, September 11, 2018 - 11:00:02 AM
Long-term archiving on: : Thursday, September 13, 2012 - 11:50:16 AM

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  • HAL Id : tel-00006847, version 1

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Hélène Delanoë-Ayari. Nucléation et croissance de contacts entre cellules biologiques. Biophysique [physics.bio-ph]. Université Joseph-Fourier - Grenoble I, 2003. Français. ⟨tel-00006847⟩

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