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Mesure du spectre de fluctuations de vésicules géantes par analyse de contours ; applications aux membranes passives et actives

Abstract : A new method of contours analysis has been developed using phase contrast microscopy on giant lipids vesicles. It allows an accurate detection in real time. We obtain fluctuations spectra and we have developed analysis to compare them with theory for planar membranes. Our method was applied to pure lipids vesicles and we obtain good agreement with theoretical predictions for fluctuations spectra. The bending rigidities deduced correspond to those of the litterature. We also study two types of non-equilibrium membranes: liposomes containing bacteriorhodopsin; our results cannot be explained by actual active membranes theory; membrane with external source of lipids were also studied, and we find a negative tension regime
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https://tel.archives-ouvertes.fr/tel-00006164
Contributor : Jacques Pécréaux <>
Submitted on : Friday, May 28, 2004 - 11:44:42 AM
Last modification on : Monday, December 14, 2020 - 9:30:09 AM
Long-term archiving on: : Wednesday, September 12, 2012 - 4:05:28 PM

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  • HAL Id : tel-00006164, version 1

Citation

Jacques Pécréaux. Mesure du spectre de fluctuations de vésicules géantes par analyse de contours ; applications aux membranes passives et actives. Biophysique [physics.bio-ph]. Université Pierre et Marie Curie - Paris VI, 2004. Français. ⟨tel-00006164⟩

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