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Conception et réalisation d'un microsystème robotisé de dépôt de liquide biologiques par microleviers en silicium pour l'élaboration de biopuces

Abstract : Biochips allow to analyze thousands of samples (DNA, proteins) in hours and therefore to know which gene responds to the action of a certain molecule or takes part of a certain disease. Our work is focused on the design and fabrication of a miniaturized and robotized silicon-cantilever-based microsystem for biological sample deposition to elaborate biochips.
After an overview of the different methods to fabricate biochips, we describe our goals. The association of a 50 nm precision positionning system and silicon micro-cantilevers was retained. The method is based on direct contact between those cantilevers and the biochip support. The deposition is made through capillary forces. The originality of our work relies on geometric dimensions (spots of a few microns in diameter), the use of minimal amount of biological samples (picoliters), enabling a lot of depositions with a single loading process. Another advantage relies on the mass production process of our cantilevers which makes our system particularly cost effective.
Our study focuses on the checking of our approach on different aspects: a biological one showing our capability to realize DNA and proteins chips and deposit various liquids with a single cantilever; a mechanical one demonstrating our system's compatibility with micro- and nano-technologies (use of cantilevers, positioning and size).
Then, the loading process of the cantilevers is highlighted: an electrowetting method is firstly used. Then dielectrophoresis which is well known to manipulate liquids is developped through the use of an electric field applied between aluminum passivated electrodes implemented along the cantilevers.
Finally piezoresistors located along the cantilevers allow the detection of the contact with the support. Therefore we have an active control of both the loading process and the deposition process in order to waranty uniformity of contact force and contact time.
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https://tel.archives-ouvertes.fr/tel-00004456
Contributor : Pascal Belaubre <>
Submitted on : Tuesday, February 3, 2004 - 3:33:06 PM
Last modification on : Friday, October 23, 2020 - 4:45:25 PM
Long-term archiving on: : Friday, April 2, 2010 - 8:13:45 PM

Identifiers

  • HAL Id : tel-00004456, version 1

Citation

Pascal Belaubre. Conception et réalisation d'un microsystème robotisé de dépôt de liquide biologiques par microleviers en silicium pour l'élaboration de biopuces. Automatique / Robotique. INSA de Toulouse, 2003. Français. ⟨tel-00004456⟩

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