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Rôle de l'interferon-gamma dans l'immunité cellulaire anti-microsporidienne. Etude du modèle de la souris déficiente pour le récepteur à l'interferon-gamma, infectée oralement avec la microsporidie Encephalitozoon intestinalis

Abstract : The microsporidium Encephalitozoon intestinalis causes gastrointestinal disease frequently associated with other clinical manifestations in immunocompromised HIV-infected patients. The intestinal mucosa provides the first physiological and immunological line of defense against the parasite. Nevertheless, most of what is known about the immunobiology of microsporidia results from studies realized with mice infected by the intraperitoneal route. Protection against these parasites has been largely associated with the cell-mediated immune response and the production of Th1-type cytokines, in particular gamma interferon (IFN-g). However, the mechanisms whereby IFN-g intervenes in the clearance of microsporidia are still not well understood. We therefore studied and compared the cellular immune response in wild-type and IFN-g receptor knockout (GRKO) mice following oral infection with E. intestinalis. First, we observed in infected wild-type mice which are able to control and eradicate the parasite, 1) the development of a Th1-type cellular immune response at the mucosal level, in the mesenteric lymph nodes and in the spleen; 2) the duodenal production of chemokines known to attract T lymphocytes and macrophages; and 3) an increase in the number of intestinal intraepithelial lymphocytes, which are able to exert cytotoxic activity against E. intestinalis-infected macrophages. In contrast, we showed in GRKO mice 1) a change in the pattern of chemokine expression at the mucocal level;2) a defect in the Th1-type cytokine expression and an earlier and/or higher expression of Th2-type cytokines; and finally 3) a less effective splenic cytotoxic T CD8+ response. Altogether, these results allow us to better understand the role of IFN-g in the antimicrosporidial immunity.
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https://tel.archives-ouvertes.fr/tel-00003434
Contributor : Nicolas Bouladoux <>
Submitted on : Tuesday, September 30, 2003 - 11:11:09 AM
Last modification on : Friday, May 29, 2020 - 4:02:16 PM
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Nicolas Bouladoux. Rôle de l'interferon-gamma dans l'immunité cellulaire anti-microsporidienne. Etude du modèle de la souris déficiente pour le récepteur à l'interferon-gamma, infectée oralement avec la microsporidie Encephalitozoon intestinalis. Immunologie. Université Pierre et Marie Curie - Paris VI, 2003. Français. ⟨tel-00003434⟩

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