. Bien-sûr, ce serait un espace numérique de toute façon, pour des raisons de stockage de résultats ; mais l'´ echelle entre pixels et microns pourrait alorsêtrealorsêtre fixe. 14. Alors que le grossissement devrait intervenir uniquement dans le modèle de système optique. 15 Transformée de Fourier

`. Modélisationmod´modélisation, . Syst-`-emesyst-`-syst-`-eme, and . Optique, la PSF n'est plusàplusà support bornée puisque sa TF l'est [Goodman 68], et on ne sait donc pas quelle taille va devoir avoir une coupe XY de l'espace objetéclairéobjetéclairé. 17 Site officiel : http://www.nr.com 4.4 TROISI, Dans le plan direct, leprobì eme de fixer une correspondance entre nombre de pixels et taille physique en micromètres est accru8µm (b) ?4.4µm (c) ?4.0µm (d) ?3.6µm (e) ?3.2µm (f) ?2.8µm (g) ?2.4µm (h) ?2.0µm (i) ?1.6µm (j) ?1.2µm (k) ?0.8µm (l) ?0.4µm (m) 0.0µm (n) 0.4µm (o), pp.0-8

. Figure, . Du, and . Web, html ANNEXE B. FICHIERS DE CONFIGURATION Object colour (if colour drawings) : green Draw objects over the image : false World volume in 2D only (default is 3D) : false Add light ray intensities : true Draw light rays not arriving to the observer : false Final intensity given for the whole ray path : false World absorption coefficient (float) : 0, World refraction index, p.1

A. Object, N. Boucher, and . Dey, 25. ;25 um de RAYON Fichier de configuration pour la bille opaque ; ; Configuration file for the ray tracing software all coordinates are world coordinates and not result volume coordinates ; (except when defining the result volume itself) ; all numerical parameters are specified for their types and the number of entries Draw rays and objects in colour (otherwise B&W) : false ; available colours are red, green and blue Light Ray colour (if colour drawings) : red Object colour (if colour drawings) : green Draw objects over the image : false World volume in 2D only (default is 3D) : false Add light ray intensities : true Draw light rays not arriving to the observer : false Final intensity given for the whole ray path : false World absorption coefficient (float) : 0. World refraction index (float) : 1.0003, 2002.

A. B. Fichiers and . Configuration, Definition of spot light matrix (orthogonal to the z axis)

A. B. Fichiers and . Configuration, variation is between [0. , 0.9[ Light ray variation of origin (float -each ray, p.25

A. Boucher and N. Dey, all coordinates are world coordinates and not result volume coordinates ; (except when defining the result volume itself) ; all numerical parameters are specified for their types and the number of entries Draw rays and objects in colour (otherwise B&W) : false ; available colours are red, green and blue Light Ray colour (if colour drawings) : red Object colour (if colour drawings) : green Draw objects over the image : false World volume in 2D only (default is 3D) : false Add light ray intensities : true Draw light rays not arriving to the observer : false Final intensity given for the whole ray path : true World absorption coefficient (float) : 0. World refraction index (float) : 1.0003, Fichier de configuration pour la bille translucide de diamètre 27µm ; ; Configuration file for the ray tracing software Definition of spot light matrix, 2002.

A. B. Fichiers and . Configuration, ray vector (z) will be vector.z +-variation (random) (once per ray) ; variation is between [0. , 0, p.25

A. Boucher and N. Dey, all coordinates are world coordinates and not result volume coordinates ; (except when defining the result volume itself) ; all numerical parameters are specified for their types and the number of entries Draw rays and objects in colour (otherwise B&W) : false ; available colours are red, green and blue Light Ray colour (if colour drawings) : red Object colour (if colour drawings) : green Draw objects over the image : false World volume in 2D only (default is 3D) : false Add light ray intensities : true Draw light rays not arriving to the observer : false Final intensity given for the whole ray path : true World absorption coefficient (float) : 0. World refraction index (float) : 1.46 ;fuchine, Definition of spot light matrix, 2002.

A. B. Fichiers and . Configuration, spot origin (z) will be origin.z +-variation (random) (once per spot)

(. V. Bove and . Michael-jr, Entropy-based depth from focus, Journal of the Optical Society of America A, vol.10, issue.4, pp.561-566, 1993.
DOI : 10.1364/JOSAA.10.000561

. De, The Influence of Astigmatism on the Response Function of an Optical System, Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, vol.233, issue.1192, pp.91-104, 1955.
DOI : 10.1098/rspa.1955.0248

. Num and . Rec, ? Numerical Recipes in C : The Art of Scientific Computing). ? A new sense for depth of field, IEEE Trans. Patt. Anal. Mach. Int, vol.9, pp.523-531, 1987.