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Mise en place et validation d'un modèle in vitro pour l'étude des propriétés mécaniques, diffusives et métaboliques d'un Foie bioartificiel à lit fluidisé.

Abstract : Today, transplantation is the only effective treatment proposed to the patients suffering from fulminant or acute hepatic failure. There is a shortage of donors worldwide resulting in high mortality rate amongst patients awaiting transplants. Hence providing a hepatic replacement device seems original. A bioartificial organ exploiting the potential performances of cells (hepatocytes in the case of a bioartificial liver) would supply the wide range of its metabolic functions. Our team proposes the concept of a fluidized bed bioreactor containing hepatocytes, stemming from the C3a human cell line, immobilized in alginate beads. The purpose of this study is to assess performances of the extra corporeal hepatic substitution device by the implementation of an in vitro model as close as possible to the in vivo situation. The presence of cells within the alginate beads has no influence on the global behavior of the fluidized bed while the use of plasma (density close to beads) can engender instability. Then, the organization, the viability and the features of hepatocytes (albumin synthesis, urea production and capacity of ammonia removal) inside the alginate beads were studied in static and dynamic conditions. In the bioreactor, hepatocytes keep their full potentialities. In the case of urea, the encapsulation seems to advance metabolism. With albumin, fluidization seems to slightly enhance the mass transfer between the encapsulated cells and the culture medium. Finally, we showed that constraints in the bioreactor only resulted in a weak alteration of the mechanical properties of beads after 6 hours of use. The use of C3a hepatocytes encapsulated into alginate beads seems thus promising for the purpose of liver supply.
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https://tel.archives-ouvertes.fr/tel-00003223
Contributor : Bertrand David <>
Submitted on : Thursday, July 31, 2003 - 11:43:34 AM
Last modification on : Wednesday, December 2, 2020 - 10:44:02 AM
Long-term archiving on: : Friday, April 2, 2010 - 8:13:18 PM

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Bertrand David. Mise en place et validation d'un modèle in vitro pour l'étude des propriétés mécaniques, diffusives et métaboliques d'un Foie bioartificiel à lit fluidisé.. Sciences du Vivant [q-bio]. Université de Technologie de Compiègne, 2002. Français. ⟨tel-00003223⟩

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