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Mécanisme d'adhérence des leucocytes aux fibres synthétiques. Application à la filtration sanguine

Abstract : INTRODUCTION. The objective of this work was to investigate the mechanisms responsible for leukocyte adhesion during blood filtration for leukoreduction. Human blood after collection is subjected to filtration to reduce the number of leukocytes present in the blood products. Blood filters are made of synthetic fibers and are designed to retain leukocytes and not erythrocytes. MATERIAL AND METHODS. To precise the different factors involved in leukocyte retention, we have set up two different experimental models: a minifilter and a flow chamber. We have compared the properties of normal leukocytes to leukemic cell lines expressing differently adhesion molecules. In addition, we studied the expression of adhesion molecules by flow cytometry and analyzed adhesion in the flow chamber using a video camera linked to a computer for image analysis. RESULTS. In the minifilter system we found that leukocyte or leukemic cell (HL60) retention was dependent upon integrin (CD11/CD18) interaction with the filter components as demonstrated by blocking experiments with specific monoclonal antibodies. The flow chamber allowed us to evaluate the adhesion to different fibers of leukocytes and leukemic cells. The HL60 and THP1 cell lines adhered more to two types of fibers (PET, PI) and this adhesion is related to integrin expression by these cells. CONCLUSION. The leukoreduction in the filter is dependent upon leukocyte adhesion to fibers. The adhesion is mediated by specific cell adhesion molecules and specific molecules present on the fibers. These results indicate that a better knowledge of the mechanism of leukocyte adhesion to synthetic material may lead to new techniques for leukoreduction of blood products.
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https://tel.archives-ouvertes.fr/tel-00002228
Contributor : Laurent Barbe <>
Submitted on : Monday, January 6, 2003 - 3:36:25 PM
Last modification on : Friday, March 27, 2020 - 2:59:27 AM
Long-term archiving on: : Friday, April 2, 2010 - 6:44:05 PM

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  • HAL Id : tel-00002228, version 1

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Laurent Barbe. Mécanisme d'adhérence des leucocytes aux fibres synthétiques. Application à la filtration sanguine. Biophysique [physics.bio-ph]. Université Paris-Diderot - Paris VII, 2001. Français. ⟨tel-00002228⟩

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