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RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates

Abstract : Liquid-liquid phase separation is thought to be a key organizing principle in eukaryotic cells to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous in vitro approaches have validated this model, yet a missing aspect is to take into consideration the complex molecular mixture and promiscuous interactions found in vivo. Here we report the versatile scaffold ArtiG to generate concentration-dependent RNA-protein condensates within living cells, as a bottom-up approach to study the impact of cosegregated endogenous components on phase separation. We demonstrate that intracellular RNA seeds the nucleation of the condensates, as it provides molecular cues to locally coordinate the formation of endogenous high-order RNP assemblies. Interestingly, the cosegregation of intracellular components ultimately impacts the size of the phase-separated condensates. Thus, RNA arises as an architectural element that can influence the composition and the morphological outcome of the condensate phases in an intracellular context.
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Submitted on : Wednesday, November 25, 2020 - 5:51:22 PM
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Marina Garcia-Jove Navarro, Shunnichi Kashida, Racha Chouaib, Sylvie Souquere, Gerard Pierron, et al.. RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates. Nature Communications, Nature Publishing Group, 2019, 10, pp.3230. ⟨10.1038/s41467-019-11241-6⟩. ⟨hal-02251322⟩



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