Développement d’une bio-encre pour la bioimpression 3D de tissus vivants : étude de la formulation et caractérisation du développement tissulaire

Abstract : This thesis focus on the development of a 3D bioprinting process for living tissue. This new field of research, 3D bioprinting, aims to fabricate tissues using a bioprinter based on the tissue engineering fundamentals.To carry out this work, a specific bioink was formulated using natural biomaterials to meet the requirement of biocompatibility, cell viability and support of a three-dimensional cellular network. Several characterizations have been used to demonstrate the cells viability during the 3D bioprinting process.The bioprinter technological evolution is then presented, starting from an open-source technology and ending with the use of a 6-axis robotic arm. The specifications of this bioprinter evolved through different prototypes.The last part of this thesis concerns tissue bioprinting results obtained through multiple collaborations. Several tissues will be studied and characterized: the dermis and its maturation towards a total skin, the cartilage and the mesenchymal stem cells bioprinting, a microvascularized tissue thanks to the incorporation of endothelial cells and finally a perfusable tissue by using a dynamic culture approach in bioreactor
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Léa Pourchet. Développement d’une bio-encre pour la bioimpression 3D de tissus vivants : étude de la formulation et caractérisation du développement tissulaire. Biotechnologie. Université de Lyon, 2018. Français. ⟨NNT : 2018LYSE1232⟩. ⟨tel-02065562⟩

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