Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

A Tandem Mass Tags (TMTs) labeling approach highlights differences between the shoot proteome of two Arabidopsis thaliana ecotypes, Col‐0 and Ws

Abstract : Arabidopsis has become a powerful model to study morphogenesis, plant growth, development but also plant response to environmental conditions. Over 1000 Arabidopsis genomes are available and show natural genetic variations. Among them, the main reference accessions Wassilewskija (Ws) and Columbia (Col-0), originally growing at contrasted altitudes and temperatures, are widely studied, but data contributing to their molecular phenotyping are still scarce. A global quantitative proteomics approach using isobaric stable isotope labeling (Tandem Mass Tags, TMT) was performed on Ws and Col-0. Plants have been hydroponically grown at 16 h/8 h (light/dark cycle) at 23°C day/19°C night for three weeks. A TMT labeling of the proteins extracted from their shoots has been performed and showed a differential pattern of protein abundance between them. These results have allowed identifying several proteins families possibly involved in the differential responses observed for Ws and Col-0 during plant development and upon environmental changes. In particular, Ws and Col-0 mainly differ in photosynthesis, cell wall-related proteins, plant defense/stress, ROS scavenging enzymes/redox homeostasis and DNA/RNA binding/transcription/translation/protein folding.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-univ-tlse3.archives-ouvertes.fr/hal-03475227
Contributeur : Alicia Benson-Rumiz Connectez-vous pour contacter le contributeur
Soumis le : vendredi 10 décembre 2021 - 16:53:05
Dernière modification le : mardi 12 avril 2022 - 11:24:01
Archivage à long terme le : : vendredi 11 mars 2022 - 19:37:34

Fichier

Leschevin _2021.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Maïté Leschevin, Paulo Marcelo, Marwa Ismael, Hélène San‐clemente, Elisabeth Jamet, et al.. A Tandem Mass Tags (TMTs) labeling approach highlights differences between the shoot proteome of two Arabidopsis thaliana ecotypes, Col‐0 and Ws. Proteomics, Wiley-VCH Verlag, 2021, 21 (11-12), pp.2000293. ⟨10.1002/pmic.202000293⟩. ⟨hal-03475227⟩

Partager

Métriques

Consultations de la notice

85

Téléchargements de fichiers

13