158 articles – 194 Notices  [english version]
Fiche concise Articles dans des revues avec comité de lecture
Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis.
Dhouib K. et al
Lab on a Chip 9, 10 (2009) 1412-21 - http://hal.archives-ouvertes.fr/hal-00442911
Kaouthar Dhouib1, Chantal Khan Malek ()2, Wilhelm Pfleging3, Bernard Gauthier-Manuel2, Roland Duffait4, Gaël Thuillier2, Rosaria Ferrigno5, Lilian Jacquamet6, Jeremy Ohana6, Jean-Luc Ferrer6, Anne Théobald-Dietrich1, Richard Giegé1, Bernard Lorber1, Claude Sauter ()1
1 :  ARN - Architecture et réactivité de l'ARN
http://www-ibmc.u-strasbg.fr/arn/
CNRS : UPR9002 – Université Louis Pasteur - Strasbourg I
Institut de Biologie Moléculaire et Cellulaire 15 rue René Descartes 67084 STRASBOURG Cedex
France
2 :  FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies
http://www.femto-st.fr
CNRS : UMR6174 – Université de Franche-Comté – Université de Technologie de Belfort-Montbeliard – Ecole Nationale Supérieure de Mécanique et des Microtechniques
32 avenue de l'Observatoire 25044 BESANCON CEDEX
France
3 :  FZK - Forschungszentrum Karlsruhe
Helmoltz
Karlsruhe
Allemagne
4 :  CTMN - Centre de Transfert des Micro et Nanotechnologies
http://www.ctmn.com
CTMN
39 Avenue de l'Observatoire, BP 1445-25007 Besançon Cedex 3, France
France
5 :  INL - Institut des nanotechnologies de Lyon - Site d'Ecully
CNRS : UMR5270 – Université Claude Bernard - Lyon I (UCBL) – Institut National des Sciences Appliquées [INSA] - Lyon – Ecole Centrale de Lyon
bat. 7 36 Av Guy de Collongue - 163 69131 ECULLY CEDEX
France
6 :  IBS - UMR 5075 - Institut de biologie structurale
http://www.ibs.fr/
CNRS : UMR5075 – CEA : DSV/IBS – Université Joseph Fourier - Grenoble I
Institut de Biologie Structurale Jean-Pierre Ebel, CEA-CNRS-UJF, 41, rue Jules Horowitz, F-38027 Grenoble, Cedex 1
France
Sciences du Vivant/Biochimie, Biologie Moléculaire/Biologie moléculaire
Sciences du Vivant/Biologie cellulaire
19417908
Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis.
Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.
Anglais

10.1039/b819362b
Lab on a Chip
internationale
21/05/2009
02/03/2009
9
10
1412-21

Crystallization; Crystallography, X-Ray; Dimethylpolysiloxanes; Macromolecular Substances; Microfluidic Analytical Techniques; Polymethyl Methacrylate