157 articles – 193 Notices  [english version]
Fiche concise Articles dans des revues avec comité de lecture
Development of a microconductometric biosniffer for detection of trimethylamine
Fillit C. et al
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 28, 5-6 (2008) 781-786 - http://hal-emse.ccsd.cnrs.fr/emse-00502010
Chrystelle Fillit ()1, Nicole Jaffrezic-Renault ()1, François Bessueille1, Didier Léonard1, Kohji Mitsubayashi2, Jacques Tardy3
1 :  SA - Sciences Analytiques
http://scanalyt.univ-lyon1.fr/
CNRS : UMR5180 – Université Claude Bernard - Lyon I (UCBL)
bat. 308 43 Bvd du 11 Novembre 1918 69622 VILLEURBANNE CEDEX
France
2 :  IBB - Institute of Biomaterials and Bioengineering
Tokyo Medical and Dental University
Japon
3 :  INL - Institut des nanotechnologies de Lyon - Site d'Ecully
CNRS : UMR5270 – Université Claude Bernard - Lyon I (UCBL) – Institut National des Sciences Appliquées [INSA] - Lyon – Ecole Centrale de Lyon
bat. 7 36 Av Guy de Collongue - 163 69131 ECULLY CEDEX
France
Sciences de l'ingénieur/Matériaux
Development of a microconductometric biosniffer for detection of trimethylamine
The paper describes the development of a conductometric biosensor for detecting trimethylamine (TMA) which is a good parameter for estimating fish freshness. The biosensor consists of two parts: a microconductometric transducer and a structured enzymatic membrane using a flavin-containing monooxygenase 3 (FMO3) which is known for its sensitivity and selectivity for TMA. The process parameters for the fabrication of the biosniffer and various experimental variables such as pH, time of exposure to glutaraldehyde vapour were investigated with regard to their influence on sensitivity and limit of detection. A low detection limit of 2 mu g/mL (ppm) and a linear range up to 40 mu g/mL (ppm) were obtained. 90% response was reached in less than 10 min. (c) 2007 Elsevier B.V. All rights reserved.
Anglais
2006

10.1016/j.msec.2007.10.020
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
internationale
01/07/2008
28
5-6
781-786

conductometric biosensor – trimethylamine – flavin-containing monooxygenase 3 – BIOSENSORS – PESTICIDES – GLUCOSE – UREA
ACL159