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MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 28, 5-6 (2008) 781-786

Development of a microconductometric biosniffer for detection of trimethylamine

Chrystelle Fillit1, Nicole Jaffrezic-Renault1, François Bessueille1, Didier Léonard1, Kohji Mitsubayashi2, Jacques Tardy3

The paper describes the development of a conductometric biosensor for detecting trimethylamine (TMA) which is a good parameter for estimating fish freshness. The biosensor consists of two parts: a microconductometric transducer and a structured enzymatic membrane using a flavin-containing monooxygenase 3 (FMO3) which is known for its sensitivity and selectivity for TMA. The process parameters for the fabrication of the biosniffer and various experimental variables such as pH, time of exposure to glutaraldehyde vapour were investigated with regard to their influence on sensitivity and limit of detection. A low detection limit of 2 mu g/mL (ppm) and a linear range up to 40 mu g/mL (ppm) were obtained. 90% response was reached in less than 10 min. (c) 2007 Elsevier B.V. All rights reserved.

1 :	SA - Sciences Analytiques
2 :	IBB - Institute of Biomaterials and Bioengineering
3 :	INL - Institut des nanotechnologies de Lyon - Site d'Ecully

mots-clés : conductometric biosensor – trimethylamine – flavin-containing monooxygenase 3 – BIOSENSORS – PESTICIDES – GLUCOSE – UREA

emse-00502010, version 1

http://hal-emse.ccsd.cnrs.fr/emse-00502010

oai:hal-emse.ccsd.cnrs.fr:emse-00502010

Contributeur : Anna Fraczkiewicz <>

Soumis le : Mardi 13 Juillet 2010, 09:38:05

Dernière modification le : Mardi 13 Juillet 2010, 09:38:05