148 articles – 175 references  [version française]
Detailed view Article in peer-reviewed journal
Methods in Molecular Biology -Clifton then Totowa- 808 (2012) 195-219
Glycoarray by DNA-directed immobilization.
François Morvan1, Yann Chevolot2, Jing Zhang3, Albert Meyer1, Sébastien Vidal4, Jean-Pierre Praly4, Jean-Jacques Vasseur1, Eliane Souteyrand2

Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the immobilization process. Herein a protocol for the synthesis of oligonucleotide glycomimetic conjugates is proposed. The resulting molecules are immobilized by hybridization on a DNA microarray (DNA-directed immobilization; DDI). DDI has been proved to be a very efficient and site-selective. This protocol provides detailed procedures for the preparation of fluorescent oligonucleotide trigalactosylmimetic conjugates and for the preparation of carbohydrate microarrays by DDI on glass slides.
1:  IBMM - Institut des Biomolécules Max Mousseron
2:  INL - Institut des nanotechnologies de Lyon - Site d'Ecully
3:  LMVI - Key Laboratory of Molecular Virology & Immunology
4:  ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires